Document Detail

Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coli.
MedLine Citation:
PMID:  22623299     Owner:  NLM     Status:  In-Data-Review    
Many carotenoid pigments are present in a small quantity in nature or low yielding from their natural sources, despite these vivid colorations. Thus, the synthesis of useful carotenoids with metabolic pathway-engineered microorganisms should offer an alternative and promising approach for their efficient production. Here, we describe a novel method for an efficient production of such carotenoids, using E. coli cells that carry heterologous mevalonate pathway-based genes. This method also enables relevant researchers to efficiently identify the function of an isolated carotenogenic gene candidate. For example, the recombinant E. coli cells, which harbor a lycopene-producing plasmid, can synthesize 12.5 mg/g dry cell weight of lycopene with the addition of lithium acetoacetate to the medium. This level corresponded to an 11.8-fold increase of that of E. coli cells carrying only the lycopene-producing plasmid.
Hisashi Harada; Norihiko Misawa
Related Documents :
11271459 - Immunology and growth characteristics of ocular basal cell carcinoma.
22067319 - Role of igfbp-3 in the regulation of β-cell mass during obesity: adipose tissue/β-cel...
19887749 - Ependymoblastoma in a japanese black heifer.
6191989 - Retinoid reversal of squamous metaplasia in organ cultures of tracheas derived from ham...
7859359 - Cell type-specific expression of human 8-oxo-7,8-dihydroguanosine triphosphatase in nor...
7208099 - Schistosoma mansoni: the sustentacular cells of the testes.
22783109 - Occurrence of clubroot on shepherd's-purse caused by plasmodiophora brassicae.
16545899 - Inducibility of udp-glucuronosyltransferase 1as by beta-naphthoflavone in hepg2 cells.
2607329 - Gonadotropin-induced luminal formation in the seminiferous tubules and efferent ductule...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  892     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  133-41     Citation Subset:  IM    
KNC Bio-Research Center, KNC Laboratories Co. Ltd., Kobe, Hyogo, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Novel zeaxanthin-producing bacteria isolated from a radioactive hot spring water.
Next Document:  Engineering Escherichia coli for Canthaxanthin and Astaxanthin Biosynthesis.