Document Detail


Novel Approach in the Biosynthesis of Functional Carotenoids in Escherichia coli.
MedLine Citation:
PMID:  22623299     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Many carotenoid pigments are present in a small quantity in nature or low yielding from their natural sources, despite these vivid colorations. Thus, the synthesis of useful carotenoids with metabolic pathway-engineered microorganisms should offer an alternative and promising approach for their efficient production. Here, we describe a novel method for an efficient production of such carotenoids, using E. coli cells that carry heterologous mevalonate pathway-based genes. This method also enables relevant researchers to efficiently identify the function of an isolated carotenogenic gene candidate. For example, the recombinant E. coli cells, which harbor a lycopene-producing plasmid, can synthesize 12.5 mg/g dry cell weight of lycopene with the addition of lithium acetoacetate to the medium. This level corresponded to an 11.8-fold increase of that of E. coli cells carrying only the lycopene-producing plasmid.
Authors:
Hisashi Harada; Norihiko Misawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  892     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  133-41     Citation Subset:  IM    
Affiliation:
KNC Bio-Research Center, KNC Laboratories Co. Ltd., Kobe, Hyogo, Japan.
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