Document Detail

Novel animal model of calvarial defect: part IV. Reconstruction of a calvarial wound complicated by durectomy.
MedLine Citation:
PMID:  23542268     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to be an effective therapy in the acute calvarial defect wound and in calvarial defects complicated by chronic scar and radiation. The authors assessed the effectiveness of rhBMP-2-mediated bone regeneration in calvarial defects complicated by durectomy.
METHODS: Sixteen adult New Zealand White rabbits underwent subtotal calvariectomy and dural removal, followed by dural repair and reconstruction in one of four groups: empty (n = 3), vehicle (buffer solution on an absorbable collagen sponge, n = 2), autologous graft (n = 3), or rhBMP-2 repair (rhBMP-2/absorbable collagen sponge, n = 8). Animals underwent computed tomographic imaging at 0, 2, 4, and 6 weeks postoperatively, followed by euthanasia and histologic analysis. Percent healing was determined by three-dimensional analysis. A 4 × 3 mixed model analysis of variance was performed on healing versus treatment group/postoperative time.
RESULTS: The rhBMP-2/absorbable collagen sponge and autograft repair groups had 51.4 and 37.3 percent healing, respectively, at 6 weeks; empty and vehicle control groups had 7.8 and 17.9 percent healing, respectively, at 6 weeks. Compared with immediate favorable reconstruction (96.8 percent healing), rhBMP-2 in this setting was significantly less effective (p = 0.001). Bone in the rhBMP-2/absorbable collagen sponge group was compact and cellular but appeared only over the intact sagittal sinus and irregularly within the absorbable collagen sponge.
CONCLUSIONS: Although promising in the acute calvarial wound and other complex defects, rhBMP-2 therapy is less effective in reconstruction following dural compromise. Future studies using additional growth factors and cell therapy may improve results in this especially difficult scenario.
Zoe M MacIsaac; Benjamin A Levine; Darren M Smith; James J Cray; Melissa Shaw; Sanjay Naran; Christopher Kinsella; Mark P Mooney; Gregory M Cooper; Joseph E Losee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plastic and reconstructive surgery     Volume:  131     ISSN:  1529-4242     ISO Abbreviation:  Plast. Reconstr. Surg.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-01     Completed Date:  2013-05-20     Revised Date:  2014-10-13    
Medline Journal Info:
Nlm Unique ID:  1306050     Medline TA:  Plast Reconstr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  512e-9e     Citation Subset:  AIM; IM    
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MeSH Terms
Bone Morphogenetic Protein 2 / therapeutic use*
Bone Regeneration / drug effects*
Disease Models, Animal*
Dura Mater / surgery*
Recombinant Proteins / therapeutic use
Skull / surgery*
Transforming Growth Factor beta / therapeutic use*
Wound Healing / drug effects*
Reg. No./Substance:
0/Bone Morphogenetic Protein 2; 0/Recombinant Proteins; 0/Transforming Growth Factor beta; 0/recombinant human bone morphogenetic protein-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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