| Novel ABA- and dehydration-inducible aldehyde dehydrogenase genes isolated from the resurrection plant Craterostigma plantagineum and Arabidopsis thaliana. | |
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MedLine Citation:
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PMID: 11849595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to identify genes that are critical for the ABA-dependent stress response in the resurrection plant Craterostigma plantagineum, a gene was isolated with homology to class 3 variable substrate aldehyde dehydrogenases (ALDH). The C. plantagineum gene Cp-ALDH constitutes a novel class of plant ALDHs. In a search for corresponding genes from Arabidopsis thaliana, Ath-ALDH3 and Ath-ALDH4 were isolated, showing 70% and 80% similarity to Cp-ALDH. Phylogenetically, the Cp- and Ath-ALDH3 and -ALDH4 proteins are closely related to aldehyde dehydrogenases from bacteria and mammalian species and are separated from known plant ALDHs and betaine-aldehyde dehydrogenases (BADH). Cp-ALDH transcript and polypeptide are up-regulated in vegetative tissues and callus in response to dehydration or ABA-treatment. Ath-ALDH3 expression was induced in response to dehydration and ABA treatment, while Ath-ALDH4 is constitutively expressed at a low level. Recombinant Cp-ALDH protein oxidizes nonanal, propionaldehyde and acetaldehyde, with Km values of 2.2 microm, 0.27 mm and 3.23 mm, respectively, in an NAD-dependent manner. Immunogold electron microscopy shows that Cp-ALDH is localized in plastids. |
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Authors:
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H H Kirch; A Nair; D Bartels |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Plant journal : for cell and molecular biology Volume: 28 ISSN: 0960-7412 ISO Abbreviation: Plant J. Publication Date: 2001 Dec |
Date Detail:
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Created Date: 2002-02-18 Completed Date: 2002-03-19 Revised Date: 2006-11-07 |
Medline Journal Info:
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Nlm Unique ID: 9207397 Medline TA: Plant J Country: England |
Other Details:
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Languages: eng Pagination: 555-67 Citation Subset: IM |
Affiliation:
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Institute of Botany, University of Bonn, Kirschallee 1, 53115 Bonn, Germany. hhkirch@uni-bonn.de |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AJ306960; AJ306961 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Abscisic Acid
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metabolism* Aldehyde Dehydrogenase / chemistry, genetics* Amino Acid Sequence Angiosperms / enzymology, genetics* Arabidopsis / enzymology, genetics* Base Sequence DNA Primers Escherichia coli / genetics Molecular Sequence Data Sequence Homology, Amino Acid Water / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 21293-29-8/Abscisic Acid; 7732-18-5/Water; EC 1.2.1.3/Aldehyde Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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