Document Detail


Novel 3D culture system for study of cardiac myocyte development.
MedLine Citation:
PMID:  12730055     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Insufficient myocardial repair after pathological processes contributes to cardiovascular disease, which is a major health concern. Understanding the molecular mechanisms that regulate the proliferation and differentiation of cardiac myocytes will aid in designing therapies for myocardial repair. Models are needed to delineate these molecular mechanisms. Here we report the development of a model system that recapitulates many aspects of cardiac myocyte differentiation that occur during early cardiac development. A key component of this model is a novel three-dimensional tubular scaffold engineered from aligned type I collagen strands. In this model embryonic ventricular myocytes undergo a transition from a hyperplastic to a quiescent phenotype, display significant myofibrillogenesis, and form critical cell-cell connections. In addition, embryonic cardiac myocytes grown on the tubular substrate have an aligned phenotype that closely resembles in vivo neonatal ventricular myocytes. We propose that embryonic cardiac myocytes grown on the tube substrate develop into neonatal cardiac myocytes via normal in vivo mechanisms. This model will aid in the elucidation of the molecular mechanisms that regulate cardiac myocyte proliferation and differentiation, which will provide important insights into myocardial development.
Authors:
Heather J Evans; Janea K Sweet; Robert L Price; Michael Yost; Richard L Goodwin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-05-01
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  285     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-15     Completed Date:  2003-08-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H570-8     Citation Subset:  IM    
Affiliation:
Department of Cell and Developmental Biology and Anatomy, University of South Carolina School of Medicine, Building 1, Rm. B-17, 6439 Garners Ferry Road, Columbia, SC 29209, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Culture Techniques / instrumentation*,  methods*
Cell Differentiation
Cell Division
Collagen Type I
Female
Heart / embryology*
Heart Ventricles / cytology,  embryology
Microfilaments / ultrastructure
Microscopy, Electron
Myocardium / cytology*
Myocytes, Cardiac / cytology*
Pregnancy
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
P20 RR 1634/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Collagen Type I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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