Document Detail

Notch signaling is involved in the regulation of Id3 gene transcription during Xenopus embryogenesis.
MedLine Citation:
PMID:  11841478     Owner:  NLM     Status:  MEDLINE    
During Xenopus embryogenesis, XId3, a member of the Id helix-loop-helix protein family, is expressed in a large variety of differentiating tissues including epidermis, cement gland, brain, neural tube, neural crest cell derivatives, somites, and tailbud. Transcription of XId3 is mediated by several cis-regulatory elements including an enhancer of 440 bp located 870 bp upstream from the transcription initiation site. The enhancer activity in embryos was studied using transgenic methodology. A galactosidase reporter gene, driven by a regulatory element composed of the enhancer and a minimal promoter derived from the XId3 gene, was expressed in transgenic embryos with a profile that faithfully reproduced that of the endogenous XId3 gene. The pattern resulted from a synergistic effect between the enhancer and the promoter, and in vitro transactivation assays showed that transcription can be stimulated by Notch signaling. The presence of potential Su(H) binding sites, in both the enhancer and the promoter, suggests that these represent candidates for in vivo cis-regulatory elements. The data presented here suggest that Notch control of differentiation may involve activation of transcription of Id, a negative regulator of bHLH transcription factors.
Sorogini Reynaud-Deonauth; Hong Zhang; Anatole Afouda; Serge Taillefert; Paul Beatus; Malgorzata Kloc; Laurence D Etkin; Jacqueline Fischer-Lougheed; Georges Spohr
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  69     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-02-13     Completed Date:  2002-05-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  198-208     Citation Subset:  IM    
Departement de Biologie Cellulaire, Université de Genève, Sciences III, 1211 Genève 4, Switzerland.
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MeSH Terms
Amino Acid Sequence
Base Sequence
DNA-Binding Proteins / biosynthesis,  genetics*
Embryo, Nonmammalian / metabolism
Enhancer Elements, Genetic
Gene Expression Regulation, Developmental*
Genes, Reporter
In Situ Hybridization
Inhibitor of Differentiation Proteins
Membrane Proteins / metabolism*
Molecular Sequence Data
Neoplasm Proteins*
Promoter Regions, Genetic
RNA, Messenger / biosynthesis
Receptors, Notch
Signal Transduction*
Transcription Factors / biosynthesis,  genetics*
Transcription, Genetic
Transcriptional Activation
Xenopus / embryology*,  genetics,  metabolism
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Inhibitor of Differentiation Proteins; 0/Membrane Proteins; 0/Neoplasm Proteins; 0/RNA, Messenger; 0/Receptors, Notch; 0/Transcription Factors; 147785-34-0/ID3 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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