| Notch signaling is essential for ventricular chamber development. | |
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MedLine Citation:
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PMID: 17336907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ventricular chamber morphogenesis, first manifested by trabeculae formation, is crucial for cardiac function and embryonic viability and depends on cellular interactions between the endocardium and myocardium. We show that ventricular Notch1 activity is highest at presumptive trabecular endocardium. RBPJk and Notch1 mutants show impaired trabeculation and marker expression, attenuated EphrinB2, NRG1, and BMP10 expression and signaling, and decreased myocardial proliferation. Functional and molecular analyses show that Notch inhibition prevents EphrinB2 expression, and that EphrinB2 is a direct Notch target acting upstream of NRG1 in the ventricles. However, BMP10 levels are found to be independent of both EphrinB2 and NRG1 during trabeculation. Accordingly, exogenous BMP10 rescues the myocardial proliferative defect of in vitro-cultured RBPJk mutants, while exogenous NRG1 rescues differentiation in parallel. We suggest that during trabeculation Notch independently regulates cardiomyocyte proliferation and differentiation, two exquisitely balanced processes whose perturbation may result in congenital heart disease. |
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Authors:
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Joaquín Grego-Bessa; Luis Luna-Zurita; Gonzalo del Monte; Victoria Bolós; Pedro Melgar; Alejandro Arandilla; Alistair N Garratt; Heesuk Zang; Yoh-Suke Mukouyama; Hanying Chen; Weinian Shou; Esteban Ballestar; Manel Esteller; Ana Rojas; José María Pérez-Pomares; José Luis de la Pompa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental cell Volume: 12 ISSN: 1534-5807 ISO Abbreviation: Dev. Cell Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-05 Completed Date: 2007-04-27 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 101120028 Medline TA: Dev Cell Country: United States |
Other Details:
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Languages: eng Pagination: 415-29 Citation Subset: IM |
Affiliation:
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Departamento de Inmunología y Oncología, Centro Nacional de Biotecnología/CSIC, Darwin 3, Campus de Cantoblanco, E-28049 Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Morphogenetic Proteins / genetics, metabolism Cell Differentiation / physiology* Cell Proliferation Ephrin-B2 / genetics, metabolism Gene Expression Regulation, Developmental / physiology Heart / embryology* Heart Ventricles / cytology, embryology, metabolism Mice Mutation / genetics Myoblasts, Cardiac / cytology, metabolism* Myocytes, Cardiac / cytology, metabolism* Nerve Tissue Proteins / genetics, metabolism Neuregulin-1 Receptor, Notch1 / genetics, metabolism Receptors, Notch / genetics, metabolism* Signal Transduction / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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P01 HL085098-01A10003/HL/NHLBI NIH HHS; R01 HL081092-01A2/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bmp10 protein, mouse; 0/Bone Morphogenetic Proteins; 0/Ephrin-B2; 0/Nerve Tissue Proteins; 0/Neuregulin-1; 0/Notch1 protein, mouse; 0/Nrg1 protein, mouse; 0/Receptor, Notch1; 0/Receptors, Notch |
| Comments/Corrections | |
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