| Not only accumulation, but also saturation status of intramuscular lipids is significantly affected by PPARγ activation. | |
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MedLine Citation:
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PMID: 22023892 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aim: Intramuscular lipid accumulation has been associated with insulin resistance, and after TZD treatment was shown to be reduced in some, but not all, studies. This work was undertaken to investigate relationships between intramuscular lipids [free fatty acids (FFA), diacylglycerols (DAG), triacylglycerol (TAG) phospholipids (PL)] and plasmalemmal fatty acid (FA) transporter expression [FAT/CD36, FABPpm] in muscles of varying oxidative capacity, after PPARγ activation (rosiglitazone) in an animal model of high fat diet induced insulin resistance. Endurance training was also included to further explore the differences in these relationships. Methods: We have used gas liquid chromatography to estimate FA content and composition in each lipid fraction. For FA transporters sarcolemmal expression, subfractionation of skeletal muscles with subsequent Western Blot technique was applied. Results: High fat diet induced intramuscular accumulation of FFA, DAG and TAG, irrespectively of muscle`s fiber composition. PPARγ activation (rosiglitazone) and to a lesser extent endurance training, further increased TAG accumulation, while reduced DAG in oxidative muscles (soleus and red gastrocnemius). Aforementioned interventions, increased also sarcolemmal FAT/CD36 and FABPpm expressions in particular muscles. Irrespectively of diet, rosiglitazone and exercise, decreased significantly FA saturation status favoring proportionate enhancement in monounsaturated fatty acid (rosiglitazone) or polyunsaturated fatty acids (endurance training). Conclusion: These findings support the conclusion that not only the change in total lipid content (DAG and TAG), but also FA composition is affected by rosiglitazone in an animal model of high fat diet induced insulin resistance. |
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Authors:
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Adrian Chabowski; Małgorzata Zendzian-Piotrowska; Artur Nawrocki; Jan Górski |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-25 |
Journal Detail:
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Title: Acta physiologica (Oxford, England) Volume: - ISSN: 1748-1716 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101262545 Medline TA: Acta Physiol (Oxf) Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Scandinavian Physiological Society. |
Affiliation:
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Department of Physiology, Medical University of Bialystok,15-22 Bialystok, Poland. |
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