Document Detail


NorthernLights in slide-based cytometry and microscopy.
MedLine Citation:
PMID:  20151452     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In recent years, slide-based cytometry has become a key technology for polychromatic cytometric investigations, and many efforts have been made to increase the number of measurable fluorochromes for multiparametric analysis. Sequential photobleaching of fluorochromes next to very photostable dyes is one approach for this technology. As the ALEXA dyes are known to be photostable as compared to the conventional fluorochromes FITC, PE (Riggs et al., Am J Pathol 1958;34:1081-1097), and APC, a differentiation within a fluorochrome pair is possible. Here, we have analyzed the newly available NorthernLights secondary antibodies for use in slide-based cytometry and microscopy. Currently, these fluorochrome-conjugates are now available with three distinct excitation- and emission maxima (NL493, NL557, NL637). Their spectral properties are similar to the frequently used fluorochromes FITC, PE, and APC and can, therefore, be used with most common excitation sources of cytometers or microscopes. As the NorthernLights are bright, resistant to photobleaching, stable in alcohols and xylene and of affordable price, these dyes are promising candidates for use with most laser- and HBO/XBA-based fluorescence microscopy-like techniques.
Authors:
J T Wessels; A C Busse; J Mahrt; B Hoffschulte; G A Mueller; A Tárnok; A Mittag
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytometry. Part A : the journal of the International Society for Analytical Cytology     Volume:  77     ISSN:  1552-4930     ISO Abbreviation:  Cytometry A     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-21     Completed Date:  2010-09-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101235694     Medline TA:  Cytometry A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  420-8     Citation Subset:  IM    
Affiliation:
Department Nephrology/Rheumatology, University Medicine, Goettingen, Germany. johannes.wessels@med.uni-goettingen.de
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MeSH Terms
Descriptor/Qualifier:
Antibodies / analysis*,  chemistry
Cell Line, Tumor
Flow Cytometry / methods*
Fluorescein-5-isothiocyanate / metabolism
Fluorescent Dyes / analysis*
Humans
Leukocytes / cytology,  radiation effects
Microscopy, Fluorescence / methods*
Photobleaching / radiation effects
Phycoerythrin / metabolism
Staining and Labeling
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Antibodies; 0/Fluorescent Dyes; 11016-17-4/Phycoerythrin; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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