Document Detail

Northeast Red Beans Produce a Thermostable and pH-Stable Defensin-Like Peptide with Potent Antifungal Activity.
MedLine Citation:
PMID:  23292358     Owner:  NLM     Status:  Publisher    
A 5.4-kDa antifungal peptide was purified from Phaseolus vulgaris L. cv. "northeast red bean" using a protocol that entailed affinity chromatography, ion exchange chromatography, and gel filtration. The molecular mass was determined by matrix-assisted laser desorption ionization time-of-flight. The N-terminal amino acid sequence of the peptide was highly homologous to defensins and defensin-like peptides from several plant species. The peptide impeded the growth of a number of pathogenic fungi, including Mycosphaerella arachidicola Khokhr. (IC(50) = 1.7 μM), Setosphaeria turcica Luttr., Fusarium oxysporum Schltdl., and Valsa mali Miyabe & G. Yamada. Antifungal activity of the peptide was fully preserved at temperatures up to 100 °C and pH values from 0 to 12. Congo red deposition at the hyphal tip of M. arachidicola was detected after exposure to the peptide, signifying that the peptide had suppressed hyphal growth. The antifungal peptide did not manifest antiproliferative activity toward human breast cancer MCF7 cells and hepatoma HepG2 cells, in contradiction to the bulk of previously reported plant defensins. The data suggest distinct structural requirements for antifungal and antiproliferative activities.
Yau Sang Chan; Tzi Bun Ng
Related Documents :
24896488 - The role of individual differences in the formation of triadic dominance orders of male...
23019158 - Structural determination of glycopeptidolipids of mycobacterium smegmatis by high-resol...
19199988 - Designer peptides: learning from nature.
234458 - Circular dichroism of c3a anaphylatoxin. effects of ph, heat, guanidinium chloride, and...
19588938 - Phospholipid-induced fibrillation of a prion amyloidogenic determinant at the air/water...
2860108 - Identification of n alpha-acetyl-alpha-endorphin and n alpha-acetyl-gamma-endorphin iso...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-6
Journal Detail:
Title:  Cell biochemistry and biophysics     Volume:  -     ISSN:  1559-0283     ISO Abbreviation:  Cell Biochem. Biophys.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9701934     Medline TA:  Cell Biochem Biophys     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Interaction of L-SIGN with Hepatitis C Virus Envelope Protein E2 Up-Regulates Raf-MEK-ERK Pathway.
Next Document:  A Hybrid Model of Tumor-Stromal Interactions in Breast Cancer.