| Norovirus-binding proteins recovered from activated sludge micro-organisms with an affinity to a noroviral capsid peptide. | |
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MedLine Citation:
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PMID: 20735509 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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AIMS: Transmission routes of noroviruses, leading aetiological agents of acute gastroenteritis, are rarely verified when outbreaks occur. Because the destination of norovirus particles being firmly captured by micro-organisms could be totally different from that of those particles moving freely, micro-organisms with natural affinity ligands such as virus-binding proteins would affect the fate of viruses in environment, if such microbial affinity ligands exist. The aim of this study is to identify norovirus-binding proteins (NoVBPs) that are presumably working as natural ligands for norovirus particles in water environments. METHODS AND RESULTS: NoVBPs were recovered from activated sludge micro-organisms by an affinity chromatography technique in which a capsid peptide of norovirus genogroup II (GII) was immobilized. The recovered NoVBPs bind to norovirus-like particles (NoVLPs) of norovirus GII, and this adsorption was stronger than that to NoVLPs of norovirus genogroup I. The profile of two-dimensional electrophoresis of NoVBPs showed that the recovered NoVBPs included at least seven spots of protein. The determination of N-terminal amino acid sequences of these NoVBPs revealed that hydrophobic interactions could contribute to the adsorption between NoVBPs and norovirus particles. CONCLUSIONS: NoVBPs conferring a high affinity to norovirus GII were successfully isolated from activated sludge micro-organisms. SIGNIFICANCE AND IMPACT OF THE STUDY: NoVBPs could be natural viral ligands and play an important role in the NoV transmission. |
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Authors:
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D Sano; K Wada; T Imai; Y Masago; T Omura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied microbiology Volume: 109 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1923-8 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal of Applied Microbiology © 2010 The Society for Applied Microbiology. |
Affiliation:
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Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, Kita-ku, Sapporo, Japan. dsano@eng.hokudai.ac.jp |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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