| Normoxic versus hyperoxic resuscitation in pediatric asphyxial cardiac arrest: effects on oxidative stress. | |
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MedLine Citation:
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PMID: 21057313 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the effects of normoxic vs. hyperoxic resuscitation on oxidative stress in a model of pediatric asphyxial cardiac arrest. DESIGN: Prospective, interventional study. SETTING: University research laboratory. SUBJECTS: Postnatal day 16-18 rats (n = 5 per group). INTERVENTIONS: Rats underwent asphyxial cardiac arrest for 9 min. Rats were randomized to receive 100% oxygen, room air, or 100% oxygen with polynitroxyl albumin (10 mL·kg⁻¹ intravenously, 0 and 30 min after resuscitation) for 1 hr from the start of cardiopulmonary resuscitation. Shams recovered in 100% oxygen or room air after surgery. MEASUREMENTS AND MAIN RESULTS: Physiological variables were recorded at baseline to 1 hr after resuscitation. At 6 hrs after asphyxial cardiac arrest, levels of reduced glutathione and protein-thiols (fluorescent assay), activities of total superoxide dismutase and mitochondrial manganese superoxide dismutase (cytochrome c reduction method), manganese superoxide dismutase expression (Western blot), and lipid peroxidation (4-hydroxynonenal Michael adducts) were evaluated in brain tissue homogenates. Hippocampal 3-nitrotyrosine levels were determined by immunohistochemistry 72 hrs after asphyxial cardiac arrest. Survival did not differ among groups. At 1 hr after resuscitation, Pao2, pH, and mean arterial pressure were decreased in room air vs. 100% oxygen rats (59 ± 3 vs. 465 ± 46 mm Hg, 7.36 ± 0.05 vs. 7.42 ± 0.03, 35 ± 4 vs. 45 ± 5 mm Hg; p < .05). Rats resuscitated with 100% oxygen had decreased hippocampal reduced glutathione levels vs. sham (15.3 ± 0.4 vs. 20.9 ± 4.1 nmol·mg protein⁻¹; p < .01). Hippocampal manganese superoxide dismutase activity was significantly increased in 100% oxygen rats vs. sham (14 ± 2.4 vs. 9.5 ± 1.6 units·mg protein⁻¹, p < .01), with no difference in protein expression of manganese superoxide dismutase. Room air and 100% oxygen plus polynitroxyl albumin groups had hippocampal reduced glutathione and manganese superoxide dismutase activity levels comparable with sham. Protein thiol levels were unchanged across groups. Compared with all other groups, rats receiving 100% oxygen had increased immunopositivity for 3-nitrotyrosine in the hippocampus and increased lipid peroxidation in the cortex. CONCLUSIONS: Resuscitation with 100% oxygen leads to increased oxidative stress in a model that mimics pediatric cardiac arrest. This may be prevented by using room air or giving an antioxidant with 100% oxygen resuscitation. |
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Authors:
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Karen H Walson; Minke Tang; Ashley Glumac; Henry Alexander; Mioara D Manole; Li Ma; Carelton J Hsia; Robert S Clark; Patrick M Kochanek; Valerian E Kagan; Hülya Bayr |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Critical care medicine Volume: 39 ISSN: 1530-0293 ISO Abbreviation: Crit. Care Med. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-20 Completed Date: 2011-03-04 Revised Date: 2012-06-26 |
Medline Journal Info:
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Nlm Unique ID: 0355501 Medline TA: Crit Care Med Country: United States |
Other Details:
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Languages: eng Pagination: 335-43 Citation Subset: AIM; IM |
Affiliation:
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Department of Critical Care Medicine, Children's Hospital of Pittsburgh, Pittsburgh, PA, USA. karen.walson@choa.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Asphyxia / complications Blood Gas Analysis Blotting, Western Disease Models, Animal Heart Arrest / etiology, mortality*, therapy* Hypoxia-Ischemia, Brain / pathology* Lipid Peroxidation Male Oxidative Stress / physiology Oxygen / therapeutic use* Oxygen Consumption / physiology Oxygen Inhalation Therapy / methods Random Allocation Rats Rats, Sprague-Dawley Reference Values Resuscitation / methods*, mortality Risk Assessment Superoxide Dismutase / metabolism Survival Rate |
| Grant Support | |
ID/Acronym/Agency:
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HD045968/HD/NICHD NIH HHS; HD057587/HD/NICHD NIH HHS; K08 HD058798/HD/NICHD NIH HHS; K08 HD058798-03/HD/NICHD NIH HHS; K08 HD058798-04/HD/NICHD NIH HHS; NS061817/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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