Document Detail

Normothermic blood perfusion of isolated rabbit kidneys. III. In vitro physiology of kidneys after perfusion with Euro-Collins solution or 7.5 M cryoprotectant (VS4).
MedLine Citation:
PMID:  12072898     Owner:  NLM     Status:  MEDLINE    
Cryopreservation of solid organs might be possible using a mixture of cell-permeable agents, cryoprotectants (CPA), which are designed to completely preclude ice crystal formation during cooling to cryogenic temperatures. The effects of a specific prototype solution (VS4) were evaluated by normothermic blood perfusion in vitro. Rabbit kidneys were divided into three groups: untreated controls ( n=7), Euro-Collins (EC)-perfused controls ( n=6) and VS4 (49%, w/v) CPA-perfused kidneys ( n=7). After a 2-h blood perfusion, five of the seven CPA-perfused kidneys developed polyuria (0.21 mlxmin(-1)xg(-1)) relative to untreated controls (0.07 mlxmin(-1)xg(-1)) or EC-perfused kidneys (0.06 mlxmin(-1)xg(-1)), owing to the lower reabsorption of water (34.3%), Na(+) (34.2%) and glucose (35.6%). Furthermore, two kidneys were non-functional with virtually no urine production. Reduced tubular function was associated with reduced oxygen consumption (3.6 versus 2.3 versus 2.0 micromolexmin(-1)xg(-1) for controls, EC- and CPA-perfused kidneys, respectively) and increased weight gain (17% versus 20% versus 30%, respectively) after blood perfusion. Therefore, the current results provide insight into both the physiological effects of VS4 and the limits of reversibility of renal pathophysiological states. Our results also indicate that in vitro monitoring of oxygen consumption and weight gain of perfused organs could be used as predictors of renal function.
Françoise G Arnaud; Bijan S Khirabadi; Gregory M Fahy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2002-05-09
Journal Detail:
Title:  Transplant international : official journal of the European Society for Organ Transplantation     Volume:  15     ISSN:  0934-0874     ISO Abbreviation:  Transpl. Int.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-19     Completed Date:  2003-01-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908516     Medline TA:  Transpl Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  278-89     Citation Subset:  IM    
Naval Medical Research Center, Resuscitative Medicine Program, 503 Robert Grant Ave, Silver Spring, MD 20910-7500, USA,
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MeSH Terms
Cryoprotective Agents / pharmacology*
Glomerular Filtration Rate
Hypertonic Solutions / pharmacology*
Kidney / blood supply,  physiology*
Organ Preservation*
Oxygen Consumption
Renal Circulation
Reg. No./Substance:
0/Cryoprotective Agents; 0/Euro-Collins' solution; 0/Hypertonic Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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