| Normalization of cholesterol homeostasis by 2-hydroxypropyl-beta-cyclodextrin in neurons and glia from Niemann-Pick C1-deficient mice. | |
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MedLine Citation:
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PMID: 22277650 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Niemann-Pick C (NPC) disease is an inherited, progressive neurodegenerative disorder caused by mutations in the NPC1 or NPC2 gene that result in an accumulation of unesterified cholesterol in late endosomes/lysosomes (LE/L) and impaired export of cholesterol from LE/L to the endoplasmic reticulum (ER). Recent studies demonstrate that administration of cyclodextrin (CD) to Npc1-/- mice eliminates cholesterol sequestration in LE/L of many tissues including the brain, delays neurodegeneration, and increases life-span of the mice. We have now investigated cholesterol homeostasis in NPC1-deficient cells of the brain in response to CD. Primary cultures of neurons and glial cells from Npc1-/- mice were incubated for 24 h with 0.1 to 10 mM CD after which survival and cholesterol homeostasis were monitored. Although 10 mM CD was profoundly neurotoxic, and altered astrocyte morphology, 0.1 and 1 mM CD were not toxic but effectively mobilized stored cholesterol from the LE/L as indicated by filipin staining. However, 0.1 and 1 mM CD altered cholesterol homeostasis in opposite directions. The data suggest that 0.1 mM CD releases cholesterol trapped in LE/L of neurons and astrocytes and increases cholesterol availability at the ER, whereas 1 mM CD primarily extracts cholesterol from the plasma membrane and reduces ER cholesterol. These studies in Npc1-/- neurons and astrocytes establish a dose of CD (0.1 mM) that would likely be beneficial in NPC disease. The findings are timely because treatment of NPC disease patients with CD is currently being initiated. |
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Authors:
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Kyle B Peake; Jean E Vance |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-25 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: - ISSN: 1083-351X ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of Alberta, Canada. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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