Document Detail

Normal relationship of beta- and non-beta-cells not needed for successful islet transplantation.
MedLine Citation:
PMID:  17563059     Owner:  NLM     Status:  MEDLINE    
Islets are composed mostly of beta-cells, and therefore stem cell research has concentrated on generating purified beta-cells, neglecting the other endocrine cell types in the islet. We investigated the presence of endocrine non-beta-cells after islet transplantation. In addition, we studied whether the transplantation of pure beta-cells, in volumes similar to that used in islet transplantation, would suffice to reverse hyperglycemia in diabetic mice. Rat islets were dispersed and beta-cells were purified by fluorescence-activated cell sorting according to their endogenous fluorescence. After reaggregation, 600 islet equivalents of the purified beta-cell aggregates were implanted into diabetic SCID mice. In mice implanted with beta-cell-enriched aggregates, the hyperglycemia was reversed and good graft function over a 12-week period was observed with regard to glucose and insulin levels, glucose tolerance tests, and graft insulin content. The endocrine cell composition of the beta-cell-enriched aggregates remained constant; before and 12 weeks after transplantation, the beta-cell-enriched aggregates comprised 95% beta-cells and 5% endocrine non-beta-cells. However, islet grafts, despite originally having comprised 75% beta-cells and 25% endocrine non-beta-cells, comprised just 5% endocrine non-beta-cells after transplantation, indicating a loss of these cells. beta-Cell-enriched aggregates can effectively reverse hyperglycemia in mice, and transplanted intact islets are depleted in non-beta-cells. It is therefore likely that islet non-beta-cells are not essential for successful islet transplantation.
Aileen J F King; Justin R Fernandes; Jennifer Hollister-Lock; Cameron E Nienaber; Susan Bonner-Weir; Gordon C Weir
Related Documents :
7828889 - Autonomous replication in vivo and in vitro of clones spanning the region of the dhfr o...
16608879 - Caveolin-1 controls cell proliferation and cell death by suppressing expression of the ...
23384419 - Targeting gemcitabine containing liposomes to cd44 expressing pancreatic adenocarcinoma...
22948659 - Pancreatic carcinoma cell lines reflect frequency and variability of cancer stem cell m...
17581699 - Densin and beta-catenin form a complex and co-localize in cultured podocyte cell juncti...
17618499 - In vitro biological evaluation of beta-tcp/hdpe--a novel orthopedic composite: a survey...
19175829 - Expression of intercellular adhesion molecule (icam)-1 or icam-2 is critical in determi...
6180089 - Isolation of two species of human lymphoblastoid (namalva) cell-derived interferon that...
10603449 - Histomorphometry of dominant and subordinate bovine ovarian follicles.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-06-11
Journal Detail:
Title:  Diabetes     Volume:  56     ISSN:  1939-327X     ISO Abbreviation:  Diabetes     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-29     Completed Date:  2007-09-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2312-8     Citation Subset:  AIM; IM    
Section on Islet Transplantation and Cell Biology, Research Division, Joslin Diabetes Center, Boston, Massachusetts 02215, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blood Glucose / metabolism
Cell Aggregation
Cell Separation
Diabetes Mellitus, Experimental / surgery
Insulin-Secreting Cells / physiology*
Islets of Langerhans / cytology,  physiology*
Islets of Langerhans Transplantation / methods,  physiology*
Mice, Inbred ICR
Rats, Sprague-Dawley
Transplantation, Heterologous
Treatment Outcome
Grant Support
Reg. No./Substance:
0/Blood Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Reduced expression of nuclear-encoded genes involved in mitochondrial oxidative metabolism in skelet...
Next Document:  Screening for insulitis in adult autoantibody-positive organ donors.