Document Detail


Normal relationship of beta- and non-beta-cells not needed for successful islet transplantation.
MedLine Citation:
PMID:  17563059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Islets are composed mostly of beta-cells, and therefore stem cell research has concentrated on generating purified beta-cells, neglecting the other endocrine cell types in the islet. We investigated the presence of endocrine non-beta-cells after islet transplantation. In addition, we studied whether the transplantation of pure beta-cells, in volumes similar to that used in islet transplantation, would suffice to reverse hyperglycemia in diabetic mice. Rat islets were dispersed and beta-cells were purified by fluorescence-activated cell sorting according to their endogenous fluorescence. After reaggregation, 600 islet equivalents of the purified beta-cell aggregates were implanted into diabetic SCID mice. In mice implanted with beta-cell-enriched aggregates, the hyperglycemia was reversed and good graft function over a 12-week period was observed with regard to glucose and insulin levels, glucose tolerance tests, and graft insulin content. The endocrine cell composition of the beta-cell-enriched aggregates remained constant; before and 12 weeks after transplantation, the beta-cell-enriched aggregates comprised 95% beta-cells and 5% endocrine non-beta-cells. However, islet grafts, despite originally having comprised 75% beta-cells and 25% endocrine non-beta-cells, comprised just 5% endocrine non-beta-cells after transplantation, indicating a loss of these cells. beta-Cell-enriched aggregates can effectively reverse hyperglycemia in mice, and transplanted intact islets are depleted in non-beta-cells. It is therefore likely that islet non-beta-cells are not essential for successful islet transplantation.
Authors:
Aileen J F King; Justin R Fernandes; Jennifer Hollister-Lock; Cameron E Nienaber; Susan Bonner-Weir; Gordon C Weir
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-06-11
Journal Detail:
Title:  Diabetes     Volume:  56     ISSN:  1939-327X     ISO Abbreviation:  Diabetes     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-29     Completed Date:  2007-09-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2312-8     Citation Subset:  AIM; IM    
Affiliation:
Section on Islet Transplantation and Cell Biology, Research Division, Joslin Diabetes Center, Boston, Massachusetts 02215, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / metabolism
Cell Aggregation
Cell Separation
Diabetes Mellitus, Experimental / surgery
Insulin-Secreting Cells / physiology*
Islets of Langerhans / cytology,  physiology*
Islets of Langerhans Transplantation / methods,  physiology*
Male
Mice
Mice, Inbred ICR
Rats
Rats, Sprague-Dawley
Transplantation, Heterologous
Treatment Outcome
Grant Support
ID/Acronym/Agency:
DK-36836/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Blood Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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