Document Detail

Normal patterning of the coronary capillary plexus is dependent on the correct transmural gradient of FGF expression in the myocardium.
MedLine Citation:
PMID:  15733666     Owner:  NLM     Status:  MEDLINE    
The formation of the coronary vessel system is vital for heart development, an essential step of which is the establishment of a capillary plexus that displays a density gradient across the myocardial wall, being higher on the epicardial than the endocardial side. This gradient in capillary plexus formation develops concurrently with transmural gradients of myocardium-derived growth factors, including FGFs. To test the role of the FGF expression gradient in patterning the nascent capillary plexus, an ectopic FGF-over-expressing site was created in the ventricular myocardial wall in the quail embryo via retroviral infection from E2-2.5, thus abolishing the transmural gradient of FGFs. In FGF virus-infected regions of the ventricular myocardium, the capillary density across the transmural axis shifted away from that in control hearts at E7. This FGF-induced change in vessel patterning was more profound at E12, with the middle zone becoming the most vascularized. An up-regulation of FGFR-1 and VEGFR-2 in epicardial and subepicardial cells adjacent to FGF virus-infected myocardium was also detected, indicating a paracrine effect on induction of vascular signaling components in coronary precursors. These results suggest that correct transmural patterning of coronary vessels requires the correct transmural expression of FGF and, therefore, FGF may act as a template for coronary vessel patterning.
David J Pennisi; Takashi Mikawa
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  279     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-28     Completed Date:  2005-06-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  378-90     Citation Subset:  IM    
Department of Cell and Developmental Biology, Cornell University Medical College, 1300 York Avenue, New York, NY 10021, USA.
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MeSH Terms
Body Patterning*
Capillaries / anatomy & histology,  embryology*
Coronary Circulation / physiology*
Embryo, Nonmammalian / anatomy & histology,  physiology
Fibroblast Growth Factors / genetics,  metabolism*
Heart / anatomy & histology,  embryology*
Morphogenesis / physiology
Myocardium / metabolism*
Paracrine Communication
Quail / anatomy & histology,  embryology
Receptor Protein-Tyrosine Kinases / genetics,  metabolism
Receptor, Fibroblast Growth Factor, Type 1
Receptors, Fibroblast Growth Factor / genetics,  metabolism
Retroviridae / genetics,  metabolism
Vascular Endothelial Growth Factor Receptor-2 / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Receptors, Fibroblast Growth Factor; 62031-54-3/Fibroblast Growth Factors; EC Protein-Tyrosine Kinases; EC, Fibroblast Growth Factor, Type 1; EC Endothelial Growth Factor Receptor-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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