Document Detail


Normal and malignant cells, including neurons, deposit plasminogen activator on the growth substrata.
MedLine Citation:
PMID:  3527728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The results of four different assay methods showed that both normal and malignant plasminogen activator-secreting cells deposited substantial amounts of this protease on tissue-culture substrata, including collagen coatings. The cells studied were Rous sarcoma virus (RSV)-transformed vole fibroblasts, a malignant neural cell line (NG108-15) capable of neurite formation, and normal mouse-regenerating sensory neurons. Deposited plasminogen activator was detected by a fibrin overlay assay at sites from which cells growing on coverslips had been gently dislodged, showing that active enzyme is left beneath cells and in the immediate pericellular area. For neuronal cells, fibrinolytic zones were detected not only at the previous positions of cell bodies but also along the terrain conditioned by neurite extension, suggesting that a trail of plasminogen activator is left behind during growth cone movement. Substratum-bound enzyme could be solubilized in buffers containing sodium dodecyl sulfate (SDS) or Triton X-100 and demonstrated by zymography following electrophoresis or assayed for amidolytic activity with a chromogenic substrate (Kabi S-2251). The results suggest that plasminogen activator may be considered a component of substrate-adhesion material. Secretory proteases deposited directly on matrix molecules would seem strategically positioned to participate in local degradation of components of the extracellular environment.
Authors:
A Krystosek; N W Seeds
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  166     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1986-09-30     Completed Date:  1986-09-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  31-46     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arvicolinae
Axons / ultrastructure
Cell Adhesion
Cell Differentiation
Cell Line
Cell Transformation, Viral
Fibrinolysis
Fibroblasts / cytology,  enzymology*
Mice
Neoplasms, Experimental / enzymology*,  pathology
Neurons / cytology,  enzymology*,  pathology
Neurons, Afferent / cytology,  enzymology
Plasminogen Activators / metabolism*
Grant Support
ID/Acronym/Agency:
CA32260/CA/NCI NIH HHS; NS-09818/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.21.-/Plasminogen Activators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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