Document Detail


Normal human follicle development: an evaluation of correlations with oestradiol, androstenedione and progesterone levels in individual follicles.
MedLine Citation:
PMID:  8849574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The mechanism of dominant follicle selection remains obscure. We have investigated the association between follicle diameter and follicular steroid levels in individual human ovarian follicles throughout the menstrual cycle. DESIGN: Fluid from ovarian follicles (n = 326) was obtained in vivo during surgery from 55 regularly cycling women with proven fertility. Follicles were divided into dominant (diameter >9 mm, n = 45) and non-dominant (diameter < or = 9 mm, n = 281) based on ultrasound measurements. MEASUREMENTS: Fluid was assayed for oestradiol (E2), androstenedione (AD), and progesterone (P). RESULTS: Median P and E2 levels were significantly lower (P < 0.0001) and AD levels significantly higher (P = 0.03) in non-dominant as compared to dominant follicles. In non-dominant follicles AD (r = 0.14, P = 0.02), but not P and E2, levels were correlated to follicular diameter, and significant changes in steroid concentrations across the menstrual cycle were absent. In dominant follicles, diameter was positively correlated with P and E2 (P < 0.001) levels, and inversely correlated with AD concentrations (P = 0.01). CONCLUSIONS: Results indicate that (1) intrafollicular oestradiol concentrations rise only in follicles exceeding 9 mm in diameter and correlate with the diameter of these dominant follicles, suggesting that significant increase in aromatase enzyme activity occurs only in the dominant follicle (2) a cycle-independent accumulation of androstenedione with size occurs in non-dominant follicles, and (3) progesterone production occurs in the largest dominant follicles only, suggesting a limited, if any, role for progesterone during follicle development.
Authors:
H J van Dessel; I Schipper; T D Pache; H van Geldorp; F H de Jong; B C Fauser
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical endocrinology     Volume:  44     ISSN:  0300-0664     ISO Abbreviation:  Clin. Endocrinol. (Oxf)     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1997-01-02     Completed Date:  1997-01-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0346653     Medline TA:  Clin Endocrinol (Oxf)     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  191-8     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, Dijkzigt Academic Hospital and Erasmus University Medical School, Rotterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Adult
Androstenedione / metabolism*
Estradiol / metabolism*
Female
Follicular Fluid / metabolism
Humans
Menstruation / metabolism
Ovarian Follicle / physiology*,  ultrasonography
Progesterone / metabolism*
Chemical
Reg. No./Substance:
50-28-2/Estradiol; 57-83-0/Progesterone; 63-05-8/Androstenedione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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