| Normal endometrial stromal cells regulate survival and apoptosis signaling through PI3K/AKt/Survivin pathway in endometrial adenocarcinoma cells in vitro. | |
| | |
MedLine Citation:
|
PMID: 21794903 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
OBJECTIVE: Stroma-tumor communication plays an important role in the genesis of neoplasia. In the current study, we investigated the effect of normal stromal cells on the survival and apoptosis signaling of endometrial cancer cells and further explored the possible mechanism implied in this communication. METHODS: Using primarily cultured normal endometrial stromal cells and an endometrial adenocarcinoma cell line, Ishikawa cells, we established a 2D-coculture system to observe the stromal cell-tumor cell crosstalk in endometrial carcinomas. Using methyl thiazolyl tetrazolium (MTT) assays, cell counting and colony formation assays, we analyzed the effect of stomal cells on the growth and proliferation of Ishikawa cells under different conditions. Using western blot analysis, we determined the effect of stromal cells on the activity of PI3K/AKt/Survivin signaling in Ishikawa cells under different conditions. Using immunohistochemistry analysis, we determined the expression of Survivin in normal endometria and endometrial adenocarcinomas. RESULTS: We found that the paracrine factors from normal endometrial stromal cells grown on Matrigel repeatedly and significantly decreased hormone-stimulated activity of PI3K/AKt/Survivin signaling in Ishikawa cells, which were proved to be increased in endometrial adenocarcinoma and essential in hormone-induced cell growth in Ishikawa cells. CONCLUSION: Paracrine factors from normal endometrial stromal cells can inhibit hormone-stimulated cell proliferation in Ishikawa cells by regulating cell survival and apoptosis through PI3K/AKt/Survivin signaling. |
| | |
Authors:
|
Min Shi; Hui Zhang; Mingjiang Li; Jing Xue; Yibing Fu; Lei Yan; Xingbo Zhao |
Related Documents
:
|
21839923 - Steep differences in wingless signaling trigger myc-independent competitive cell intera... 21724843 - Essential role of endocytosis of the type ii transmembrane serine protease tmprss6 in r... 2150753 - Trans-dominant suppressor mutations of the h-ras oncogene. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-7-25 |
Journal Detail:
|
Title: Gynecologic oncology Volume: - ISSN: 1095-6859 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
|
Created Date: 2011-7-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0365304 Medline TA: Gynecol Oncol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier Inc. All rights reserved. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Morbidity, mortality and overall survival in elderly women undergoing primary surgical debulking for...
Next Document: IL-22 attenuates IL-25 production by lung epithelial cells and inhibits antigen-induced eosinophilic...