Document Detail


Normal mouse intestinal epithelial cells as a model for the in vitro invasion of Trichinella spiralis infective larvae.
MedLine Citation:
PMID:  22066026     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been known for many years that Trichinella spiralis initiates infection by penetrating the columnar epithelium of the small intestine; however, the mechanisms used by the parasite in the establishment of its intramulticellular niche in the intestine are unknown. Although the previous observations indicated that invasion also occurs in vitro when the infective larvae are inoculated onto cultures of intestinal epithelial cells (e.g., human colonic carcinoma cell line Caco-2, HCT-8), a normal readily manipulated in vitro model has not been established because of difficulties in the culture of primary intestinal epithelial cells (IECs). In this study, we described a normal intestinal epithelial model in which T. spiralis infective larvae were shown to invade the monolayers of normal mouse IECs in vitro. The IECs derived from intestinal crypts of fetal mouse small intestine had the ability to proliferate continuously and express specific cytokeratins as well as intestinal functional cell markers. Furthermore, they were susceptible to invasion by T. spiralis. When inoculated onto the IEC monolayer, infective larvae penetrated cells and migrated through them, leaving trails of damaged cells heavily loaded with T. spiralis larval excretory-secretory (ES) antigens which were recognized by rabbit immune sera on immunofluorescence test. The normal intestinal epithelial model of invasion mimicking the natural environment in vivo will help us to further investigate the process as well as the mechanisms by which T. spiralis establishes its intestinal niche.
Authors:
Hui Jun Ren; Jing Cui; Zhong Quan Wang; Ruo Dan Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-10-31
Journal Detail:
Title:  PloS one     Volume:  6     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2011  
Date Detail:
Created Date:  2011-11-08     Completed Date:  2012-03-15     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e27010     Citation Subset:  IM    
Affiliation:
Department of Parasitology, Medical College, Zhengzhou University, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Proliferation
Cell Separation
Cells, Cultured
Epithelial Cells / parasitology*,  pathology*
Fluorescent Antibody Technique
Humans
Intestines / pathology*
Larva / physiology
Mice
Mice, Inbred BALB C
Microscopy, Phase-Contrast
Models, Biological*
Rabbits
Staining and Labeling
Trichinella spiralis / physiology*
Trichinellosis / parasitology*,  pathology*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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