Document Detail

Norepinephrine induces apoptosis in skin melanophores by attenuating cAMP-PKA signals via alpha2-adrenoceptors in the medaka, Oryzias latipes.
MedLine Citation:
PMID:  11601657     Owner:  NLM     Status:  MEDLINE    
The density of skin melanophores in many teleost fish decreases during long-term adaptation to a white background. Using the medaka, Oryzias latipes, we previously reported that apoptosis is responsible for the decrease in melanophores, and that a sympathetic neurotransmitter, norepinephrine (NE), induces their apoptosis in skin tissue cultures. In this study, we show that NE-induced apoptosis of melanophores is mediated by the activation of alpha2-adrenoceptors. Clonidine, an alpha2-adrenoceptor agonist, induced apoptotic melanophore death in skin organ culture, while phenylephrine, an alpha1-adrenoceptor agonist, had no effect. NE-induced apoptosis was diminished by an alpha2-adrenoceptor antagonist, yohimbine, but an alpha1-adrenoceptor antagonist, prazosin, did not abrogate the effect of NE. Furthermore, forskolin inhibited NE-induced apoptosis, while an inhibitor of PKA, H-89, mimicked the effect of NE. These results suggest that NE induces apoptosis in melanophores by attenuating cAMP-PKA signaling via alpha2-adrenoceptors.
N Uchida-Oka; M Sugimoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society     Volume:  14     ISSN:  0893-5785     ISO Abbreviation:  Pigment Cell Res.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-16     Completed Date:  2002-04-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8800247     Medline TA:  Pigment Cell Res     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  356-61     Citation Subset:  IM    
Department of Biomolecular Science, Faculty of Science, Toho University, Funabashi, Chiba, Japan.
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MeSH Terms
Adrenergic alpha-Agonists / pharmacology
Adrenergic alpha-Antagonists / pharmacology
Apoptosis / drug effects*,  physiology
Clonidine / pharmacology
Culture Techniques
Cyclic AMP / metabolism*
Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors,  metabolism*
Cysteine Proteinase Inhibitors / pharmacology
Forskolin / pharmacology
Isoquinolines / pharmacology
Melanophores / drug effects*,  metabolism
Norepinephrine / pharmacology*
Oligopeptides / pharmacology
Oryzias / physiology*
Phenylephrine / pharmacology
Prazosin / pharmacology
Protein Isoforms
Receptors, Adrenergic, alpha-2 / metabolism*
Signal Transduction / physiology
Skin / cytology,  metabolism
Skin Pigmentation / physiology
Yohimbine / pharmacology
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Adrenergic alpha-Antagonists; 0/Cysteine Proteinase Inhibitors; 0/Isoquinolines; 0/Oligopeptides; 0/Protein Isoforms; 0/Receptors, Adrenergic, alpha-2; 0/Sulfonamides; 0/aspartyl-glutamyl-valyl-aspartal; 127243-85-0/H 89; 146-48-5/Yohimbine; 19216-56-9/Prazosin; 4205-90-7/Clonidine; 51-41-2/Norepinephrine; 59-42-7/Phenylephrine; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; EC AMP-Dependent Protein Kinases

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