Document Detail


Norepinephrine is coreleased with serotonin in mouse taste buds.
MedLine Citation:
PMID:  19052199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ATP and serotonin (5-HT) are neurotransmitters secreted from taste bud receptor (type II) and presynaptic (type III) cells, respectively. Norepinephrine (NE) has also been proposed to be a neurotransmitter or paracrine hormone in taste buds. Yet, to date, the specific stimulus for NE release in taste buds is not well understood, and the identity of the taste cells that secrete NE is not known. Chinese hamster ovary cells were transfected with alpha(1A) adrenoceptors and loaded with fura-2 ("biosensors") to detect NE secreted from isolated mouse taste buds and taste cells. Biosensors responded to low concentrations of NE (>or=10 nm) with a reliable fura-2 signal. NE biosensors did not respond to stimulation with KCl or taste compounds. However, we recorded robust responses from NE biosensors when they were positioned against mouse circumvallate taste buds and the taste buds were stimulated with KCl (50 mm) or a mixture of taste compounds (cycloheximide, 10 microm; saccharin, 2 mm; denatonium, 1 mm; SC45647, 100 microm). NE biosensor responses evoked by stimulating taste buds were reversibly blocked by prazosin, an alpha(1A) receptor antagonist. Together, these findings indicate that taste bud cells secrete NE when they are stimulated. We isolated individual taste bud cells to identify the origin of NE release. NE was secreted only from presynaptic (type III) taste cells and not receptor (type II) cells. Stimulus-evoked NE release depended on Ca(2+) in the bathing medium. Using dual biosensors (sensitive to 5-HT and NE), we found all presynaptic cells secrete 5-HT and 33% corelease NE with 5-HT.
Authors:
Yijen A Huang; Yutaka Maruyama; Stephen D Roper
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  28     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-04     Completed Date:  2009-01-12     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13088-93     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, and Program in Neuroscience, University of Miami School of Medicine, Miami, Florida 33136, USA.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic alpha-Antagonists / pharmacology
Animals
CHO Cells
Chemoreceptor Cells / drug effects,  metabolism,  secretion*
Cricetinae
Cricetulus
Cycloheximide / pharmacology
Fura-2
Indicators and Reagents
Mice
Mice, Inbred C57BL
Mice, Transgenic
Norepinephrine / metabolism,  pharmacology,  secretion*
Potassium Chloride / pharmacology
Quaternary Ammonium Compounds / pharmacology
Receptors, Adrenergic, alpha-1 / genetics
Saccharin / pharmacology
Serotonin / metabolism*
Synaptic Transmission / drug effects,  physiology*
Taste / drug effects,  physiology
Taste Buds / drug effects,  metabolism,  secretion*
Tongue / innervation,  physiology*
Grant Support
ID/Acronym/Agency:
5R01DC007630/DC/NIDCD NIH HHS; R01 DC007630/DC/NIDCD NIH HHS; R01 DC007630-02/DC/NIDCD NIH HHS; R01 DC007630-03/DC/NIDCD NIH HHS
Chemical
Reg. No./Substance:
0/Adra1a protein, mouse; 0/Adrenergic alpha-Antagonists; 0/Indicators and Reagents; 0/Quaternary Ammonium Compounds; 0/Receptors, Adrenergic, alpha-1; 103735-41-7/denatonium saccharide; 50-67-9/Serotonin; 51-41-2/Norepinephrine; 66-81-9/Cycloheximide; 7447-40-7/Potassium Chloride; 81-07-2/Saccharin; 96314-98-6/Fura-2
Comments/Corrections

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