|Norepinephrine is coreleased with serotonin in mouse taste buds.|
|PMID: 19052199 Owner: NLM Status: MEDLINE|
|ATP and serotonin (5-HT) are neurotransmitters secreted from taste bud receptor (type II) and presynaptic (type III) cells, respectively. Norepinephrine (NE) has also been proposed to be a neurotransmitter or paracrine hormone in taste buds. Yet, to date, the specific stimulus for NE release in taste buds is not well understood, and the identity of the taste cells that secrete NE is not known. Chinese hamster ovary cells were transfected with alpha(1A) adrenoceptors and loaded with fura-2 ("biosensors") to detect NE secreted from isolated mouse taste buds and taste cells. Biosensors responded to low concentrations of NE (>or=10 nm) with a reliable fura-2 signal. NE biosensors did not respond to stimulation with KCl or taste compounds. However, we recorded robust responses from NE biosensors when they were positioned against mouse circumvallate taste buds and the taste buds were stimulated with KCl (50 mm) or a mixture of taste compounds (cycloheximide, 10 microm; saccharin, 2 mm; denatonium, 1 mm; SC45647, 100 microm). NE biosensor responses evoked by stimulating taste buds were reversibly blocked by prazosin, an alpha(1A) receptor antagonist. Together, these findings indicate that taste bud cells secrete NE when they are stimulated. We isolated individual taste bud cells to identify the origin of NE release. NE was secreted only from presynaptic (type III) taste cells and not receptor (type II) cells. Stimulus-evoked NE release depended on Ca(2+) in the bathing medium. Using dual biosensors (sensitive to 5-HT and NE), we found all presynaptic cells secrete 5-HT and 33% corelease NE with 5-HT.|
|Yijen A Huang; Yutaka Maruyama; Stephen D Roper|
Related Documents :
|19884319 - Cell-to-cell communication in intact taste buds through atp signalling from pannexin 1 ...
12372779 - Phosphatase inhibitors increase the open probability of enac in a6 cells.
22361929 - Establishment and characterization of six human gastric carcinoma cell lines, including...
2373159 - New techniques for the study of lens electrophysiology.
20619629 - Extracellular recording of glycine receptor chloride channel activity as a prototype fo...
1705969 - Development of serotonin-induced ion currents in identified embryonic retzius cells fro...
1671059 - Aerobic radiosensitization by sr 4233 in rodent and human cells: mechanistic and therap...
8265349 - Modified binding of proteins from calcitonin-negative tumor cells to the neuroendocrine...
2253329 - Hippocampal neurotrophic factors influence the perikaryal size of septal acetylcholines...
|Type: Journal Article; Research Support, N.I.H., Extramural|
|Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 28 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2008 Dec|
|Created Date: 2008-12-04 Completed Date: 2009-01-12 Revised Date: 2014-09-14|
Medline Journal Info:
|Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States|
|Languages: eng Pagination: 13088-93 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Chemoreceptor Cells / drug effects, metabolism, secretion*
Cycloheximide / pharmacology
Indicators and Reagents
Mice, Inbred C57BL
Norepinephrine / metabolism, pharmacology, secretion*
Potassium Chloride / pharmacology
Quaternary Ammonium Compounds / pharmacology
Receptors, Adrenergic, alpha-1 / genetics
Saccharin / pharmacology
Serotonin / metabolism*
Synaptic Transmission / drug effects, physiology*
Taste / drug effects, physiology
Taste Buds / drug effects, metabolism, secretion*
Tongue / innervation, physiology*
|5R01DC007630/DC/NIDCD NIH HHS; R01 DC007630/DC/NIDCD NIH HHS; R01 DC007630-02/DC/NIDCD NIH HHS; R01 DC007630-03/DC/NIDCD NIH HHS|
|0/Adra1a protein, mouse; 0/Adrenergic alpha-Antagonists; 0/Indicators and Reagents; 0/Quaternary Ammonium Compounds; 0/Receptors, Adrenergic, alpha-1; 333DO1RDJY/Serotonin; 660YQ98I10/Potassium Chloride; 98600C0908/Cycloheximide; FST467XS7D/Saccharin; G2NT7SMW7M/denatonium saccharide; TSN3DL106G/Fura-2; X4W3ENH1CV/Norepinephrine|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Olfactory information processing in the Drosophila antennal lobe: anything goes?
Next Document: Activity-dependent dynamic microtubule invasion of dendritic spines.