| Norepinephrine is coreleased with serotonin in mouse taste buds. | |
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MedLine Citation:
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PMID: 19052199 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ATP and serotonin (5-HT) are neurotransmitters secreted from taste bud receptor (type II) and presynaptic (type III) cells, respectively. Norepinephrine (NE) has also been proposed to be a neurotransmitter or paracrine hormone in taste buds. Yet, to date, the specific stimulus for NE release in taste buds is not well understood, and the identity of the taste cells that secrete NE is not known. Chinese hamster ovary cells were transfected with alpha(1A) adrenoceptors and loaded with fura-2 ("biosensors") to detect NE secreted from isolated mouse taste buds and taste cells. Biosensors responded to low concentrations of NE (>or=10 nm) with a reliable fura-2 signal. NE biosensors did not respond to stimulation with KCl or taste compounds. However, we recorded robust responses from NE biosensors when they were positioned against mouse circumvallate taste buds and the taste buds were stimulated with KCl (50 mm) or a mixture of taste compounds (cycloheximide, 10 microm; saccharin, 2 mm; denatonium, 1 mm; SC45647, 100 microm). NE biosensor responses evoked by stimulating taste buds were reversibly blocked by prazosin, an alpha(1A) receptor antagonist. Together, these findings indicate that taste bud cells secrete NE when they are stimulated. We isolated individual taste bud cells to identify the origin of NE release. NE was secreted only from presynaptic (type III) taste cells and not receptor (type II) cells. Stimulus-evoked NE release depended on Ca(2+) in the bathing medium. Using dual biosensors (sensitive to 5-HT and NE), we found all presynaptic cells secrete 5-HT and 33% corelease NE with 5-HT. |
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Authors:
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Yijen A Huang; Yutaka Maruyama; Stephen D Roper |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 28 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-04 Completed Date: 2009-01-12 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 13088-93 Citation Subset: IM |
Affiliation:
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Department of Physiology and Biophysics, and Program in Neuroscience, University of Miami School of Medicine, Miami, Florida 33136, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenergic alpha-Antagonists
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pharmacology Animals CHO Cells Chemoreceptor Cells / drug effects, metabolism, secretion* Cricetinae Cricetulus Cycloheximide / pharmacology Fura-2 Indicators and Reagents Mice Mice, Inbred C57BL Mice, Transgenic Norepinephrine / metabolism, pharmacology, secretion* Potassium Chloride / pharmacology Quaternary Ammonium Compounds / pharmacology Receptors, Adrenergic, alpha-1 / genetics Saccharin / pharmacology Serotonin / metabolism* Synaptic Transmission / drug effects, physiology* Taste / drug effects, physiology Taste Buds / drug effects, metabolism, secretion* Tongue / innervation, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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5R01DC007630/DC/NIDCD NIH HHS; R01 DC007630/DC/NIDCD NIH HHS; R01 DC007630-02/DC/NIDCD NIH HHS; R01 DC007630-03/DC/NIDCD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adra1a protein, mouse; 0/Adrenergic alpha-Antagonists; 0/Indicators and Reagents; 0/Quaternary Ammonium Compounds; 0/Receptors, Adrenergic, alpha-1; 103735-41-7/denatonium saccharide; 50-67-9/Serotonin; 51-41-2/Norepinephrine; 66-81-9/Cycloheximide; 7447-40-7/Potassium Chloride; 81-07-2/Saccharin; 96314-98-6/Fura-2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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