Document Detail

Noradrenaline induces supersensitivity of adenylyl cyclase in NG108-15 and HT29-18 cells but not in the beta-cell RINm5F.
MedLine Citation:
PMID:  7662513     Owner:  NLM     Status:  MEDLINE    
In some cells a supersensitive adenylyl cyclase response follows hormonal inhibition of the enzyme. We have compared the effect of pre-treatment with noradrenaline in RINm5F cells to that in cells known to demonstrate supersensitivity. In NG108-15 cells, 12 or 24 hours pre-treatment with 1-10 microM noradrenaline markedly enhanced forskolin (1 microM) stimulated cAMP accumulation compared to that in untreated cells (12 h, 429 +/- 123 vs 72 +/- 8 pmol/mg protein; 24 h 190 +/- 32 vs 82 +/- 10 for treated and untreated cells, respectively). In a second cell, HT29-18, 30 min pre-treatment with 10 microM noradrenaline enhanced forskolin (10 microM) stimulated cAMP accumulation from 47 +/- 13 pmol/mg protein to 405 +/- 109 pmol/mg protein. In contrast, pre-treatment of RINm5F cells with noradrenaline under the same conditions did not enhance the forskolin response. These data indicate that noradrenaline which induces a supersensitive adenylyl cyclase response in NG108-15 and HT29-18 cells does not induce the response in the insulin secreting cell RINm5F.
A M McDermott; G W Sharp
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular signalling     Volume:  7     ISSN:  0898-6568     ISO Abbreviation:  Cell. Signal.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-10-10     Completed Date:  1995-10-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8904683     Medline TA:  Cell Signal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  277-85     Citation Subset:  IM    
Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
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MeSH Terms
Adenylate Cyclase / antagonists & inhibitors,  metabolism*
Cell Line
Cyclic AMP / metabolism
Dose-Response Relationship, Drug
Forskolin / pharmacology
Gastric Inhibitory Polypeptide / pharmacology
Hybrid Cells
Norepinephrine / pharmacology*
Pancreatic Neoplasms
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
51-41-2/Norepinephrine; 59392-49-3/Gastric Inhibitory Polypeptide; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; EC Cyclase

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