| Nonylphenol enhances apoptosis induced by serum deprivation in PC12 cells. | |
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MedLine Citation:
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PMID: 14987954 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although nonylphenol is well known as an endocrine disrupting chemical, there is little information concerning biological effect of nonylphenol. In this study, we investigated effect of nonylphenol on apoptosis induced by serum deprivation in PC12 cells using TUNEL and DNA fragmentation assays. In addition, changes in contents of proapoptotic factors, Bad and Bax, and antiapoptotic factor, Bcl-2, and enzyme activity of caspase-3 were studied. Below 100 ng/ml of nonylphenol increased TUNEL signals, DNA fragmentation and content of proapoptotic factor, Bad as compared to those by serum deprivation without nonylphenol. Furthermore, addition of nonylphenol enhanced caspase-3 activity and Z-VAD, caspase-3 inhibitor, diminished such effect. These results indicated that below 100 ng/ml of nonylphenol enhanced apoptosis induced by serum deprivation via caspase-3 activation in PC12 cell. |
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Authors:
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Miho Aoki; Masaaki Kurasaki; Takeshi Saito; Sayaka Seki; Toshiyuki Hosokawa; Yasumitsu Takahashi; Hiroyoshi Fujita; Toshio Iwakuma |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Life sciences Volume: 74 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-02-27 Completed Date: 2004-03-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
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Languages: eng Pagination: 2301-12 Citation Subset: IM |
Affiliation:
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Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Kita-10, Nishi-5, Kita, Sapporo 060-0810, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Caspases / metabolism Culture Media, Serum-Free DNA Fragmentation Electrophoresis, Agar Gel In Situ Nick-End Labeling PC12 Cells Phenols / metabolism, toxicity* Rats |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free; 0/Phenols; 25154-52-3/nonylphenol; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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