Document Detail


Nonvectorial surface transport, endocytosis via a Di-leucine-based motif, and bidirectional transcytosis of chimera encoding the cytosolic tail of rat FcRn expressed in Madin-Darby canine kidney cells.
MedLine Citation:
PMID:  10085147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transfer of passive immunity from the mother to the fetus or newborn involves the transport of IgG across several epithelia. Depending on the species, IgG is transported prenatally across the placenta and yolk sac or is absorbed from colostrum and milk by the small intestine of the suckling newborn. In both cases apical to basolateral transepithelial transport of IgG is thought to be mediated by FcRn, an IgG Fc receptor with homology to major histocompatibility class I antigens. Here, we analyzed the intracellular routing of chimera encoding the rat FcRn tail fused to the ecto- and transmembrane domain of the macrophage FcgammaRIIb. Newly synthesized chimera were delivered in a nonvectorial manner to the apical and basolateral cell surface, from where the chimera were able to internalize and transcytose. Apical to basolateral and basolateral to apical transcytosis were differently regulated. This intracellular routing of the chimera is similar to that of the native FcRn, indicating that the cytosolic tail of the receptor is necessary and sufficient to endow an unrelated FcR with the intracellular transport behavior of FcRn. Furthermore, the di-leucine motif in the cytosolic domain of FcRn was required for rapid and efficient endocytosis but not for basolateral sorting of the chimera.
Authors:
I Stefaner; A Praetor; W Hunziker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  274     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-04-29     Completed Date:  1999-04-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  8998-9005     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry, University of Lausanne, BIL Biomedical Research Center, 155 Ch. des Boveresses, 1066 Epalinges, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antigens, CD / genetics*,  metabolism
Biological Transport / genetics
Cell Line
Dogs
Endocytosis / genetics*
Histocompatibility Antigens Class I
Immunoglobulin Fab Fragments / analysis
Immunohistochemistry
Macrophages / metabolism
Molecular Sequence Data
Rats
Receptors, Fc / genetics*,  metabolism
Receptors, IgG / genetics*,  metabolism
Recombinant Fusion Proteins / genetics*,  metabolism
Transfection / genetics
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Fc gamma receptor IIB; 0/Fc receptor, neonatal; 0/Histocompatibility Antigens Class I; 0/Immunoglobulin Fab Fragments; 0/Receptors, Fc; 0/Receptors, IgG; 0/Recombinant Fusion Proteins

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