Document Detail


Nontypeable Haemophilus influenzae in the lower respiratory tract of patients with chronic bronchitis.
MedLine Citation:
PMID:  11739144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The frequency of colonization and intracellular localization of nontypeable Haemophilus influenzae (NTHi) in the lower respiratory tract was determined in healthy adults and in clinically stable and acutely ill chronic bronchitis (CB) patients. NTHi was recovered from bronchial wash or bronchial brush specimens in 6 of 23 (26%) stable CB patients and in 1 of 15 (7%) CB patients with a respiratory exacerbation. No NTHi (0 of 26) was recovered from lower tract specimens of healthy adults undergoing anesthesia for elective surgery. Molecular typing of NTHi strains revealed that five of nine patients with stable CB had different strains in upper respiratory tract and bronchial wash/brush specimens collected simultaneously. Four stable patients with CB had different strains recovered on repeat bronchoscopy. These results demonstrate the frequent colonization of the lower airways of stable CB patients with multiple strains of NTHi. Bronchial biopsies also were examined for intracellular NTHi by in situ hybridization and immunofluorescence microscopy. Intracellular NTHi were found in 0 of 7 healthy adults, 8 of 24 patients with clinically stable CB, and 13 of 15 acutely ill CB patients. This observation suggests a role for intracellular infection by NTHi in the pathogenesis of exacerbations of CB.
Authors:
V Bandi; M A Apicella; E Mason; T F Murphy; A Siddiqi; R L Atmar; S B Greenberg
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  164     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-12     Completed Date:  2002-01-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2114-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Adult
Aged
Biopsy
Bronchitis, Chronic / complications*
Bronchoalveolar Lavage
Bronchoscopy
Carrier State / microbiology*
Case-Control Studies
DNA, Bacterial / analysis,  genetics
Female
Fluorescent Antibody Technique
Haemophilus Infections / complications*,  microbiology*
Haemophilus influenzae / classification*,  genetics
Humans
In Situ Hybridization
Male
Middle Aged
Phenotype
Polymerase Chain Reaction
Ribotyping
Sputum / microbiology
Grant Support
ID/Acronym/Agency:
AI 19641/AI/NIAID NIH HHS; AI 24616/AI/NIAID NIH HHS; N01AI 65298/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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