Document Detail


Nontransformed, GM-CSF-dependent macrophage lines are a unique model to study tissue macrophage functions.
MedLine Citation:
PMID:  23708119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Macrophages are diverse cell types in the first line of antimicrobial defense. Only a limited number of primary mouse models exist to study their function. Bone marrow-derived, macrophage-CSF-induced cells with a limited life span are the most common source. We report here a simple method yielding self-renewing, nontransformed, GM-CSF/signal transducer and activator of transcription 5-dependent macrophages (Max Planck Institute cells) from mouse fetal liver, which reflect the innate immune characteristics of alveolar macrophages. Max Planck Institute cells are exquisitely sensitive to selected microbial agents, including bacterial LPS, lipopeptide, Mycobacterium tuberculosis, cord factor, and adenovirus and mount highly proinflammatory but no anti-inflammatory IL-10 responses. They show a unique pattern of innate responses not yet observed in other mononuclear phagocytes. This includes differential LPS sensing and an unprecedented regulation of IL-1α production upon LPS exposure, which likely plays a key role in lung inflammation in vivo. In conclusion, Max Planck Institute cells offer an useful tool to study macrophage biology and for biomedical science.
Authors:
György Fejer; Mareike Dorothee Wegner; Ildiko Györy; Idan Cohen; Peggy Engelhard; Elena Voronov; Thomas Manke; Zsolt Ruzsics; Lars Dölken; Olivia Prazeres da Costa; Nora Branzk; Michael Huber; Antje Prasse; Robert Schneider; Ron N Apte; Chris Galanos; Marina A Freudenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-05-24
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  110     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-12     Completed Date:  2013-08-23     Revised Date:  2013-12-12    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E2191-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Cells / cytology,  immunology*,  metabolism
Cell Differentiation / genetics,  immunology
Cell Proliferation / drug effects
Cells, Cultured
Cytokines / genetics,  immunology,  metabolism
Granulocyte-Macrophage Colony-Stimulating Factor / immunology*,  pharmacology
Humans
Interleukin-1alpha / genetics,  immunology,  metabolism
Lipopolysaccharides / immunology,  pharmacology
Macrophages / cytology,  immunology*,  metabolism
Macrophages, Alveolar / cytology,  immunology*,  metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Mycobacterium tuberculosis / immunology
Oligonucleotide Array Sequence Analysis
Phagocytosis / immunology
Propionibacterium acnes / immunology
STAT5 Transcription Factor / genetics,  immunology,  metabolism
Transcriptome / drug effects,  genetics,  immunology
Chemical
Reg. No./Substance:
0/Cytokines; 0/Interleukin-1alpha; 0/Lipopolysaccharides; 0/STAT5 Transcription Factor; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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