Document Detail


Nonthermal ATP-dependent fluctuations contribute to the in vivo motion of chromosomal loci.
MedLine Citation:
PMID:  22517744     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosomal loci jiggle in place between segregation events in prokaryotic cells and during interphase in eukaryotic nuclei. This motion seems random and is often attributed to brownian motion. However, we show here that locus dynamics in live bacteria and yeast are sensitive to metabolic activity. When ATP synthesis is inhibited, the apparent diffusion coefficient decreases, whereas the subdiffusive scaling exponent remains constant. Furthermore, the magnitude of locus motion increases more steeply with temperature in untreated cells than in ATP-depleted cells. This "superthermal" response suggests that untreated cells have an additional source of molecular agitation, beyond thermal motion, that increases sharply with temperature. Such ATP-dependent fluctuations are likely mechanical, because the heat dissipated from metabolic processes is insufficient to account for the difference in locus motion between untreated and ATP-depleted cells. Our data indicate that ATP-dependent enzymatic activity, in addition to thermal fluctuations, contributes to the molecular agitation driving random (sub)diffusive motion in the living cell.
Authors:
Stephanie C Weber; Andrew J Spakowitz; Julie A Theriot
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-04-19
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-09     Completed Date:  2012-08-13     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7338-43     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
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MeSH Terms
Descriptor/Qualifier:
2,4-Dinitrophenol / pharmacology
Adenosine Triphosphate / metabolism*
Algorithms
Antimetabolites / pharmacology
Chromosomes, Bacterial / genetics*,  metabolism
Chromosomes, Fungal / genetics*,  metabolism
Deoxyglucose / pharmacology
Diffusion
Enzyme Inhibitors / pharmacology
Escherichia coli / drug effects,  genetics,  metabolism
Genetic Loci*
Kinetics
Microscopy, Video
Motion
Nucleic Acid Synthesis Inhibitors / pharmacology
Rifampin / pharmacology
Saccharomyces cerevisiae / drug effects,  genetics,  metabolism
Sodium Azide / pharmacology
Temperature
Time-Lapse Imaging
Uncoupling Agents / pharmacology
Grant Support
ID/Acronym/Agency:
AI-67712/AI/NIAID NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Antimetabolites; 0/Enzyme Inhibitors; 0/Nucleic Acid Synthesis Inhibitors; 0/Uncoupling Agents; 13292-46-1/Rifampin; 154-17-6/Deoxyglucose; 26628-22-8/Sodium Azide; 51-28-5/2,4-Dinitrophenol; 56-65-5/Adenosine Triphosphate
Comments/Corrections
Comment In:
Proc Natl Acad Sci U S A. 2012 May 8;109(19):7138-9   [PMID:  22562796 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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