Document Detail


Nontargeted metabolomics reveals biochemical pathways altered in response to captivity and food limitation in the freshwater mussel Amblema plicata.
MedLine Citation:
PMID:  25463058     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Effective conservation of freshwater mussels (Mollusca: Bivalvia: Unionidae), one of the most endangered groups of animals in North America, is compromised by limited knowledge of their health. We address this gap in knowledge by characterizing the metabolic profile of Amblema plicata in the wild and in response to captivity and food limitation. Eight mussels brought into captivity from the wild were isolated for 18days without a food source. Hemolymph samples were taken prior to, and 9 and 18days after the start of the experiment; these samples were analyzed by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. We detected and identified 71 biochemicals in the hemolymph of freshwater mussels; of these, 49 showed significant changes during captivity and/or food limitation (p<0.05). Fasting resulted in severe metabolite depletion. Captive (but fed) mussels experienced changes similar to (albeit less severe than) fasting mussels, suggesting that mussels may experience nutritional deficiency under common captive conditions. A. plicata responded to food limitation stress by preferentially using energy reserves for maintenance rather than growth. Carbohydrate and energy metabolism exhibited down-regulation in captive, food-limited, and wild mussels. Lipid metabolism was up-regulated in captive/food-limited mussels and unchanged in wild mussels. Amino acid metabolism was up-regulated in wild mussels and down-regulated in captive/food-limited mussels. Nucleotide metabolism was up-regulated in the wild mussels, down-regulated in food-limited mussels, and unchanged in captive mussels. The different responses between treatment groups suggest potential for nucleotide metabolism as a biomarker of health status for freshwater mussels.
Authors:
Ieva Roznere; G Thomas Watters; Barbara A Wolfe; Marymegan Daly
Related Documents :
17044888 - Prevalence and intensity of oestrus ovis in akkaraman sheep in the konya region of turkey.
24275588 - The effect of partial food deprivation on the astroglia in the dorsal subnucleus of the...
425288 - Mass poisoning in cattle, palm doves and mink caused by the coccidiostat dibutyltin dil...
23518828 - Oxytocin, feeding, and satiety.
17451668 - Vitrification of human laser treated blastocysts within cut standard straws (css): nove...
23489518 - Merging dietary assessment with the adolescent lifestyle.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-10-6
Journal Detail:
Title:  Comparative biochemistry and physiology. Part D, Genomics & proteomics     Volume:  12C     ISSN:  1878-0407     ISO Abbreviation:  Comp. Biochem. Physiol. Part D Genomics Proteomics     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-12-2     Completed Date:  -     Revised Date:  2014-12-3    
Medline Journal Info:
Nlm Unique ID:  101270611     Medline TA:  Comp Biochem Physiol Part D Genomics Proteomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  53-60     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Host-defense peptides from skin secretions of Fraser's clawed frog Xenopus fraseri (Pipidae): Furthe...
Next Document:  Characterization of the genomic responses in early Senegalese sole larvae fed diets with different d...