Document Detail

Nonsymbiotic hemoglobins in rice are synthesized during germination and in differentiating cell types.
MedLine Citation:
PMID:  11770429     Owner:  NLM     Status:  MEDLINE    
Nonsymbiotic hemoglobins (ns-Hbs) previously have been found in monocots and dicots; however, very little is known about the tissue and cell type localization as well as the physiological function(s) of these oxygen-binding proteins. We report the immunodetection and immunolocalization of ns-Hbs in rice (Oryza sativa L.) by Western blotting and in situ confocal laser scanning techniques. Ns-Hbs were detected in soluble extracts of different tissues from the developing rice seedling by immunoblotting. Levels of ns-Hbs increased in the germinating seed for the first six days following imbibition and remained relatively constant thereafter. In contrast, ns-Hb levels decreased during leaf maturation. Roots and mesocotyls contained detectable, but low levels of ns-Hbs. Split-seed experiments revealed that ns-Hbs are synthesized de novo during seed germination and are expressed in the absence of any signal originating from the embryo. Immunolocalization of ns-Hbs by confocal microscopy indicated the presence of ns-Hbs primarily in differentiated and differentiating cell types of the developing seedling, such as the aleurone, scutellum, root cap cells, sclerenchyma, and tracheary elements. To our knowledge, this is the first report of the specific cellular localization of these proteins during seedling development.
E J Ross; L Shearman; M Mathiesen; Y J Zhou; R Arredondo-Peter; G Sarath; R V Klucas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Protoplasma     Volume:  218     ISSN:  0033-183X     ISO Abbreviation:  Protoplasma     Publication Date:  2001  
Date Detail:
Created Date:  2001-12-27     Completed Date:  2002-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  125-33     Citation Subset:  IM    
Department of Biochemistry, George W. Beadle Center, University of Nebraska-Lincoln, Lincoln, NE 68588-0664, USA.
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MeSH Terms
Blotting, Western
Cell Differentiation*
Cell Extracts
Hemoglobins / analysis,  biosynthesis*
Microscopy, Confocal
Oryza sativa / cytology*,  metabolism*,  physiology
Plant Proteins / analysis,  biosynthesis
Seeds / cytology,  metabolism
Reg. No./Substance:
0/Cell Extracts; 0/Hemoglobins; 0/Plant Proteins

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