| Nonspecific protease and elastase activities in rat leukocytes. | |
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MedLine Citation:
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PMID: 7049937 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Extracts were prepared from rat peritoneal leukocytes obtained 4 h after glycogen injection and assayed for proteolytic enzyme activities against various substrates. The substrates used included acid-denatured bovine hemoglobin, bovine serum albumin, a partially purified preparation of rat pulmonary basement membrane, bovine neck ligament elastin, and an artificial substrate with elastase specificity. A high level of activity was observed when hemoglobin was used as the substrate. The serum albumin and basement membrane preparation were also readily hydrolyzed by the leukocyte extract. In contrast, the native elastin and synthetic elastase substrate were much more resistant. Although the leukocyte extract demonstrated little intrinsic elastase activity, when it was mixed with a commercial hog pancreatic elastase preparation, it greatly potentiated the elastolytic activity, suggesting the activation of a latent enzyme. |
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Authors:
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J Varani; D Ward; K J Johnson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Inflammation Volume: 6 ISSN: 0360-3997 ISO Abbreviation: Inflammation Publication Date: 1982 Jun |
Date Detail:
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Created Date: 1982-10-12 Completed Date: 1982-10-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7600105 Medline TA: Inflammation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 177-87 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascitic Fluid / cytology Hemoglobins / metabolism Hydrolysis Leukocytes / enzymology* Pancreatic Elastase / blood* Peptide Hydrolases / blood* Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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CA29550/CA/NCI NIH HHS; CA29551/CA/NCI NIH HHS; HL00889/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hemoglobins; EC 3.4.-/Peptide Hydrolases; EC 3.4.21.36/Pancreatic Elastase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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