Document Detail


Nonself recognition is mediated by HET-C heterocomplex formation during vegetative incompatibility.
MedLine Citation:
PMID:  12234924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nonself recognition during vegetative growth in filamentous fungi is mediated by heterokaryon incompatibility (het) loci. In Neurospora crassa, het-c is one of 11 het loci. Three allelic specificity groups, termed het-c(OR), het-c(PA) and het-c(GR), exist in natural populations. Heterokaryons or partial diploids that contain het-c alleles of alternative specificity show severe growth inhibition, repression of conidiation and hyphal compartmentation and death (HCD). Using epitope-tagged HET-C, we show that nonself recognition is mediated by the presence of a heterocomplex composed of polypeptides encoded by het-c alleles of alternative specificity. The HET-C heterocomplex localized to the plasma membrane (PM); PM-bound HET-C heterocomplexes occurred in all three het-c incompatible allelic interactions. Strains containing het-c constructs deleted for a predicted signal peptide sequence formed HET-C heterocomplexes in the cytoplasm and showed a growth arrest phenotype. Our finding is a step towards understanding nonself recognition mechanisms that operate during vegetative growth in filamentous fungi, and provides a model for investigating relationships between recognition mechanisms and cell death.
Authors:
Sovan Sarkar; Gopal Iyer; Jennifer Wu; N Louise Glass
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The EMBO journal     Volume:  21     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-17     Completed Date:  2002-11-08     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  4841-50     Citation Subset:  IM    
Affiliation:
Plant and Microbial Biology Department, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Amino Acid Sequence
Cell Fractionation
Cell Membrane / metabolism*
Fungal Proteins / genetics,  metabolism*
Genes, Reporter
Hyphae / cytology,  metabolism
Macromolecular Substances
Membrane Proteins / genetics,  metabolism
Molecular Sequence Data
Neurospora crassa / cytology,  physiology*
Recombinant Fusion Proteins / genetics,  metabolism
Transformation, Genetic
Grant Support
ID/Acronym/Agency:
GM60468-01/GM/NIGMS NIH HHS; R01 GM060468/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fungal Proteins; 0/Macromolecular Substances; 0/Membrane Proteins; 0/Recombinant Fusion Proteins
Comments/Corrections

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