| Nonself recognition is mediated by HET-C heterocomplex formation during vegetative incompatibility. | |
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MedLine Citation:
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PMID: 12234924 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nonself recognition during vegetative growth in filamentous fungi is mediated by heterokaryon incompatibility (het) loci. In Neurospora crassa, het-c is one of 11 het loci. Three allelic specificity groups, termed het-c(OR), het-c(PA) and het-c(GR), exist in natural populations. Heterokaryons or partial diploids that contain het-c alleles of alternative specificity show severe growth inhibition, repression of conidiation and hyphal compartmentation and death (HCD). Using epitope-tagged HET-C, we show that nonself recognition is mediated by the presence of a heterocomplex composed of polypeptides encoded by het-c alleles of alternative specificity. The HET-C heterocomplex localized to the plasma membrane (PM); PM-bound HET-C heterocomplexes occurred in all three het-c incompatible allelic interactions. Strains containing het-c constructs deleted for a predicted signal peptide sequence formed HET-C heterocomplexes in the cytoplasm and showed a growth arrest phenotype. Our finding is a step towards understanding nonself recognition mechanisms that operate during vegetative growth in filamentous fungi, and provides a model for investigating relationships between recognition mechanisms and cell death. |
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Authors:
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Sovan Sarkar; Gopal Iyer; Jennifer Wu; N Louise Glass |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The EMBO journal Volume: 21 ISSN: 0261-4189 ISO Abbreviation: EMBO J. Publication Date: 2002 Sep |
Date Detail:
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Created Date: 2002-09-17 Completed Date: 2002-11-08 Revised Date: 2010-09-14 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England |
Other Details:
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Languages: eng Pagination: 4841-50 Citation Subset: IM |
Affiliation:
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Plant and Microbial Biology Department, 111 Koshland Hall, University of California, Berkeley, CA 94720, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Amino Acid Sequence Cell Fractionation Cell Membrane / metabolism* Fungal Proteins / genetics, metabolism* Genes, Reporter Hyphae / cytology, metabolism Macromolecular Substances Membrane Proteins / genetics, metabolism Molecular Sequence Data Neurospora crassa / cytology, physiology* Recombinant Fusion Proteins / genetics, metabolism Transformation, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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GM60468-01/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Macromolecular Substances; 0/Membrane Proteins; 0/Recombinant Fusion Proteins |
| Comments/Corrections | |
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