Document Detail


Nonresonant Raman imaging of protein distribution in single human cells.
MedLine Citation:
PMID:  12400086     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A confocal Raman microscope is used to study the protein distribution inside biological cells. It is shown that high quality Raman imaging of the protein distribution can be obtained using confocal nonresonant Raman imaging (lambda(exc) = 647.1 nm). The results are shown for two different human cell types. Perpheral blood lymphocytes are used as an example of the fully maturated cells with a low level of nuclear transcription. Human eye lens epithelial cells are used as an example of cells with a high level of nuclear activity. The protein distribution in both cell types is completely different. The nuclear distribution of the protein largely varies in the peripheral blood lymphocyte cells, while proteins are more homogenously distributed over the nuclear space in the eye lens epithelial cells. The imaging time is approximately 20 min for a field of view of 10 x 10 microm(2). The size of the sampling volume is 1.4 fL using a full width at half-maximum criterion along the z axis and a 1/e(2) criterion in the xy plane. The results presented here indicate that Raman imaging is particularly of interest in the study of cellular processes, like phagocytosis, apoptosis, chromatin compaction, and cellular differentiation, which are accompanied by relatively large-scale redistributions of the materials.
Authors:
N Uzunbajakava; A Lenferink; Y Kraan; B Willekens; G Vrensen; J Greve; C Otto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biopolymers     Volume:  72     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2003  
Date Detail:
Created Date:  2002-10-25     Completed Date:  2004-01-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley Periodicals, Inc.
Affiliation:
Biomedical Technology Institute, Department of Applied Physics, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands. n.uzunbajakava@tn.utwente.nl
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / chemistry*
Epithelial Cells / chemistry,  cytology
Humans
Lens, Crystalline / chemistry,  cytology
Lymphocytes / chemistry,  cytology
Microscopy, Confocal / methods*
Proteins / analysis*
Spectrum Analysis, Raman / methods*
Chemical
Reg. No./Substance:
0/Proteins

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