Document Detail


Nonradioactive ligand binding assay for epidermal growth factor receptor.
MedLine Citation:
PMID:  2221374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid and sensitive nonradioactive ligand binding assay for identifying the agonists and antagonists of epidermal growth factor receptor is described. The assay is easy to perform, has a within-assay error of less than 15%, and does not use radioactive substances. Nonspecific binding is less than 10% and the assay is sensitive enough to detect epidermal growth factor (EGF) antagonists at as little as 10 nM, if the antagonist has the same affinity as EGF. The assay is carried out in 96-well plates and is particularly suitable for large-scale screening. This report includes description of the methods that we used to assess the validity of the assay system, and the results of that assessment.
Authors:
I C King; J J Catino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  188     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-11-07     Completed Date:  1990-11-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  97-100     Citation Subset:  IM    
Affiliation:
Department of Experimental Therapeutics, Bristol-Myers Squibb Company, Wallingford, Connecticut 06492.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / analysis
Biotin / diagnostic use
Cells, Cultured
Epidermal Growth Factor / metabolism
Humans
Iodine Radioisotopes / diagnostic use
Ligands
Receptor, Epidermal Growth Factor / analysis*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Iodine Radioisotopes; 0/Ligands; 58-85-5/Biotin; 62229-50-9/Epidermal Growth Factor; EC 2.7.10.1/Receptor, Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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