| Nonpregnant sheep uterine type I and type III nitric oxide synthase expression is differentially regulated by estrogen. | |
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MedLine Citation:
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PMID: 10208984 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to characterize the effect of estrogen on the expression of neuronal and endothelial isoforms of nitric oxide (NO) synthase (NOS) in myometrium, endometrium, and caruncle (nonglandular endometrium) in nonpregnant sheep. Twenty sheep were castrated during synchronized estrus (Days 14-16) and 4 days after surgery treated i.v. through the jugular with 100 microg/day of estradiol-17beta for 5 (n = 6) or 8 (n = 6) days or with vehicle (n = 8). Nitric oxide synthase mRNA was measured by ribonuclease protection assay, and NOS protein mass was measured by Western immunoblotting. Data were analyzed by ANOVA and Tukey's test. The three distinct uterine compartments studied contained the mRNA and protein for the neuronal (type I NOS) and the endothelial (type III NOS) isoforms of NOS. However, no inducible NOS was detected. Estrogen exhibited a differential effect on NOS expression in a tissue compartment- and NOS isoform-specific manner. In myometrium and caruncles, but not in endometrium, type I NOS mRNA and protein mass increased significantly (p < 0.05) after 5 or 8 days of estrogen. In contrast, type III NOS increased significantly in myometrium only after 8 days, whereas in endometrium and caruncles the increase was significant in the 5-day treatment group (p < 0.05). We conclude that the expression of type I NOS and type III NOS in the uterus are differentially regulated by estrogen. This differential regulation suggests that the NO produced within the uterus serves more than one physiological role. In myometrium it may be a uterorelaxant and regulate glucose utilization, and in endometrium and myometrium it may regulate blood flow. |
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Authors:
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J Zhang; G A Massmann; C P Mirabile; J P Figueroa |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biology of reproduction Volume: 60 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1999 May |
Date Detail:
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Created Date: 1999-06-03 Completed Date: 1999-06-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1198-203 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Perinatal Research Laboratory, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Endometrium / drug effects, enzymology Estradiol / pharmacology Estrogens / pharmacology* Female Glucose / metabolism Myometrium / drug effects, enzymology Nitric Oxide Synthase / biosynthesis* Nitric Oxide Synthase Type II Nitric Oxide Synthase Type III RNA, Messenger / biosynthesis Ribonucleases / analysis, metabolism Sheep Uterus / drug effects, enzymology* |
| Grant Support | |
ID/Acronym/Agency:
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HD-32542/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Estrogens; 0/RNA, Messenger; 50-28-2/Estradiol; 50-99-7/Glucose; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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