Document Detail


Nonoxidative metabolism of 2-butoxyethanol via fatty acid conjugation in Fischer 344 rats.
MedLine Citation:
PMID:  8968408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nonoxidative metabolism of ethylene glycol monobutyl ether (2-butoxyethanol or BE) via fatty acid conjugation was studied in the liver of Fischer 344 male rats following a single oral administration of 500 mg/kg body weight [ethyl-1,2-14C]BE (70 microCi/kg). Animals were killed 2 h after the treatment, hepatic lipids extracted, and the neutral lipids were separated using solid-phase extraction. The neutral lipid fraction was subjected to preparative thin-layer chromatography, and the esters corresponding to the relative flow of authentic fatty acid 2-butoxyethyl esters were recovered and analyzed by reversed-phase high-performance liquid chromatography (HPLC) using methanol-water (37:3, v/v) as solvent. Approximately 85% of the 14C label present in the ester fraction was coeluted at retention times corresponding to the different fatty acid 2-butoxyethyl ester standards. The radioactive fractions were analyzed by electron impact mass spectrometry. Molecular ion peaks and fragmentation patterns similar to that of 16:0, 18:0, 18:1, 18:2, and 20:4 fatty acid 2-butoxyethyl ester standards were detected in the corresponding radioactive HPLC fractions. Fatty acid ethyl ester synthase (FAEES), purified from the rat liver microsomal fraction, was also found to catalyze the formation of 18:1 fatty acid 2-butoxyethyl ester. These studies demonstrate that BE is metabolized nonoxidatively via conjugation with long-chain fatty acids, and the formation of these esters appears to be catalyzed by the enzyme(s) involved in fatty acid conjugation of xenobiotic alcohols. However, the biological significance of BE conjugation with fatty acids remains to be investigated.
Authors:
B S Kaphalia; B I Ghanayem; G A Ansari
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of toxicology and environmental health     Volume:  49     ISSN:  0098-4108     ISO Abbreviation:  J Toxicol Environ Health     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-14     Completed Date:  1997-01-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7513622     Medline TA:  J Toxicol Environ Health     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  463-79     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0609, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyltransferases / metabolism
Administration, Oral
Animals
Carbon Radioisotopes / metabolism
Chromatography, High Pressure Liquid / methods
Esters / analysis,  chemical synthesis,  chemistry*
Ethylene Glycols / administration & dosage,  metabolism*
Fatty Acids / metabolism*
Liver / chemistry,  metabolism
Male
Oxidation-Reduction
Rats
Rats, Inbred F344
Solvents / administration & dosage,  metabolism*
Grant Support
ID/Acronym/Agency:
ES 04815/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Esters; 0/Ethylene Glycols; 0/Fatty Acids; 0/Solvents; 111-76-2/n-butoxyethanol; EC 2.3.-/Acyltransferases; EC 3.1.1.67/fatty acyl ethyl ester synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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