Document Detail


Noninvasive redox fluorometry: how light can be used to monitor alterations of corneal mitochondrial function.
MedLine Citation:
PMID:  6690223     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ultraviolet light can result in corneal, lenticular and retinal damage; however it can also be used (at much lower intensities) to measure the light induced alteration of cellular respiration and function. Mitochondrial function can be measured by noninvasive redox fluorometry which measures the intrinsic mitochondrial fluorescence of the reduced pyridine nucleotides (NADH + NADPH) and of the oxidized flavoproteins. Impaired mitochondrial respiration results in an increase in the reduced pyridine nucleotide fluorescence signal (366 nm excitation and 450 nm emission) and in a decrease in the oxidized flavoprotein fluorescence signal (450 nm excitation and 550 nm emission). These redox signals are sensitive to the cellular supply and utilization of oxygen and glucose as well as the mitochondrial work load. The effects of a reduced oxygen supply to the corneal epithelial surface can be measured. While redox fluorometry has been applied to the study of corneal hypoxia, it may also be used to monitor the effects of light induced damage to the lens and the retina. Noninvasive redox fluorometry is a sensitive technique to measure the effects of light on mitochondrial function in ocular tissue.
Authors:
B R Masters
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Current eye research     Volume:  3     ISSN:  0271-3683     ISO Abbreviation:  Curr. Eye Res.     Publication Date:  1984 Jan 
Date Detail:
Created Date:  1984-02-24     Completed Date:  1984-02-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8104312     Medline TA:  Curr Eye Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  23-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cornea / metabolism*
Epithelium / metabolism
Fluorometry
Male
Mitochondria / metabolism*
Oxidation-Reduction
Rabbits
Ultraviolet Rays / diagnostic use
Grant Support
ID/Acronym/Agency:
EY-02421/EY/NEI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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