Document Detail


Noninvasive measurement of three-dimensional morphology of adhered animal cells employing phase-shifting laser microscope.
MedLine Citation:
PMID:  17994898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Noninvasive measurement of 3-D morphology of adhered animal cells employing a phase-shifting laser microscope (PLM) is investigated, in which the phase shift for each pixel in the view field caused by cell height and the difference in refractive indices between the cells and the medium is determined. By employing saline with different refractive indices instead of a culture medium, the refractive index of the cells, which is necessary for the determination of cell height, is determined under PLM. The observed height of Chinese hamster ovary (CHO) cells cultivated under higher osmolarity is lower than that of the cells cultivated under physiological osmolarity, which is in agreement with previous data observed under an atomic force microscope (AFM). Maximum heights of human bone marrow mesenchymal stem cells and human umbilical cord vein endothelial cells measured under PLM and AFM agree well with each other. The maximum height of nonadherent spherical CHO cells observed under PLM is comparable to the cell diameter measured under a phase contrast inverted microscope. Laser irradiation, which is necessary for the observation under PLM, did not affect 3-D cell morphology. In conclusion, 3-D morphology of adhered animal cells can be noninvasively measured under PLM.
Authors:
Mutsumi Takagi; Takayuki Kitabayashi; Syunsuke Ito; Masashi Fujiwara; Akio Tokuda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  12     ISSN:  1083-3668     ISO Abbreviation:  J Biomed Opt     Publication Date:    2007 Sep-Oct
Date Detail:
Created Date:  2007-11-12     Completed Date:  2008-01-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  054010     Citation Subset:  IM    
Affiliation:
Hokkaido University, Graduate School of Engineering, Division of Biotechnology and Macromolecular Chemistry, Sapporo 060-8628, Japan. takagi-m@eng.hokudai.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion / physiology*
Cell Size*
Cells, Cultured
Equipment Design
Equipment Failure Analysis
Humans
Image Interpretation, Computer-Assisted / methods*
Imaging, Three-Dimensional / methods*
Microscopy, Confocal / instrumentation*,  methods
Microscopy, Phase-Contrast / instrumentation*,  methods
Refractometry / instrumentation*,  methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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