Document Detail

Noninvasive measurement of exhaled nitric oxide in a spontaneously breathing mouse.
MedLine Citation:
PMID:  11316645     Owner:  NLM     Status:  MEDLINE    
Nitric oxide (NO) has been detected in the exhaled gas of animals and humans. In previous work, investigators have used anesthetized, mechanically ventilated animals to obtain exhaled NO (E(NO)) measurements, which has unclear effects on the levels of E(NO) and does not allow for repeated analysis of E(NO). We sought to measure E(NO) from a single, spontaneously breathing mouse. The mouse was placed in a small Plexiglas chamber and allowed to acclimatize before exhaled gas was collected for E(NO) analysis. Under optimal operating conditions of flow and pressure, the mean concentration of exhaled NO (FE(NO)) of 25 mice was 10.1 +/- 1.0 ppb. The maximal variation of FE(NO) when repeatedly measured daily in individual animals was 2.1 ppb. Administration of L-NAME, a nonselective NOS inhibitor, reduced FE(NO) by 51 +/- 6% (p < 0.01). Intraperitoneally administered lipopolysaccharide induced acute lung injury and increased FE(NO) by 30 +/- 7% (p < 0.05). We have demonstrated that it is possible to noninvasively measure E(NO) from a single, spontaneously breathing mouse. This novel technique provides a stable, reproducible, and responsive measure of E(NO) in mice. This technique will be of use in determining cellular and isoform sources of E(NO), as well as the role of endogenous NO in lung disease.
S Weicker; T A Karachi; J A Scott; D G McCormack; S Mehta
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  163     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-04-24     Completed Date:  2001-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1113-6     Citation Subset:  AIM; IM    
A.C. Burton Vascular Biology Laboratory, London Health Science Centre, London, Ontario, Canada.
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MeSH Terms
Analysis of Variance
Anesthetics / pharmacology
Atmosphere Exposure Chambers
Breath Tests / methods*
Chemiluminescent Measurements
Mice, Inbred C57BL
Monitoring, Physiologic / methods*
Nitric Oxide / metabolism*
Respiratory Distress Syndrome, Adult / metabolism
Reg. No./Substance:
0/Anesthetics; 10102-43-9/Nitric Oxide

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