| Noninvasive identification of subcellular organization and nuclear morphology features associated with leukemic cells using light-scattering spectroscopy. | |
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MedLine Citation:
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PMID: 21456879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leukemia is the most common and deadly cancer among children and one of the most prevalent cancers among adults. Improvements in its diagnosis and monitoring of leukemic patients could have a significant impact in their long-term treatment. We demonstrate that light-scattering spectroscopy (LSS)-based approaches could serve as a tool to achieve this goal. Specifically, we characterize the light scattering properties of leukemic (NALM-6) cells and compare them to those of normal lymphocytes and granulocytes in the 440-710 nm range, over ±4 deg about the exact backscattering direction. We find that the LSS spectra are well described by an inverse power-law wavelength dependence, with a power exponent insensitive to the scattering angle but significantly higher for leukemic cells than for normal leukocytes. This is consistent with differences in the subcellular morphology of these cells, detected in differential interference contrast images. Furthermore, the residual light-scattering signal, extracted after subtracting the inverse power-law fit from the data, can be analyzed assuming a Gaussian distribution of spherical scatterers using Mie theory. This analysis yields scatterer sizes that are consistent with the diameters of cell nuclei and allows the detection of the larger nuclei of NALM-6 cells compared to those of lymphocytes and granulocytes. |
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Authors:
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Austin Hsiao; Martin Hunter; Cherry Greiner; Sharad Gupta; Irene Georgakoudi |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of biomedical optics Volume: 16 ISSN: 1560-2281 ISO Abbreviation: J Biomed Opt Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-04-04 Completed Date: 2011-08-09 Revised Date: 2012-03-29 |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: United States |
Other Details:
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Languages: eng Pagination: 037007 Citation Subset: IM |
Affiliation:
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Tufts University, Biomedical Engineering Department, Medford, Massachusetts 02115, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Cell Nucleus / pathology* Cellular Structures / pathology Fractals Granulocytes / cytology Humans Light Lymphocytes / cytology Microscopy, Interference Models, Biological Optical Phenomena Precursor Cell Lymphoblastic Leukemia-Lymphoma / diagnosis, pathology* Reference Values Scattering, Radiation* Spectrum Analysis / methods* |
| Grant Support | |
ID/Acronym/Agency:
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R21CA114684/CA/NCI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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