Document Detail


Noninvasive colorectal cancer screening.
MedLine Citation:
PMID:  12064797     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Abnormal mucin with the STn epitope is produced by colorectal cancer cells and is immunologically distinguishable from normal colonic mucin. Herein we describe a technique of detecting abnormal mucin in fecal samples as a method of screening for colorectal neoplasia. Soluble glycoproteins from fecal samples of patients with symptoms of bowel disease and asymptomatic volunteer subjects were isolated by centrifugation and ethanol precipitation. The protein content of the soluble fraction was normalized and tested by immunoassay (slot dot). Anti-COTA monoclonal antibody SP-21, which reacts with STn epitope, was applied in the reaction, and the optical density of each slot dot was determined by imaging densitometer. Quantitative values of samples were determined from the standard curve obtained with highly purified COTA. COTA values > 15 microg/ml were considered positive for neoplasia. Results indicated that 5/6 colon cancers, 6/22 adenomas, 1/8 colitis cases, and 2/58 normal patients were positive in the test. The pilot study revealed that COTA assay is sensitive and more specific than Hemoccult screening for colorectal neoplasia.
Authors:
Keshab D Pant; John D McCracken
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Digestive diseases and sciences     Volume:  47     ISSN:  0163-2116     ISO Abbreviation:  Dig. Dis. Sci.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-14     Completed Date:  2002-06-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902782     Medline TA:  Dig Dis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1236-40     Citation Subset:  AIM; IM    
Affiliation:
Department of Gastroenterology, Loma Linda Medical Center, California 92350, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Antigens, Neoplasm / analysis*
Colonic Neoplasms / diagnosis*,  immunology
Colonoscopy
Feces / chemistry*
Humans
Immunoenzyme Techniques*
Mucins / analysis*
Pilot Projects
Risk Factors
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Neoplasm; 0/Mucins; 0/colon-ovarian tumor antigen, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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