Document Detail

Noninvasive blood pressure measurement in mice using pulsed Doppler ultrasound.
MedLine Citation:
PMID:  12706189     Owner:  NLM     Status:  MEDLINE    
Existing tail-cuff pressure devices for mice use tail flow sensors that measure only systolic and mean pressure. We developed a method to obtain systolic and diastolic pressure in mice using a pulsed Doppler flow velocity sensor and a tail-cuff and validated the method against pressure signals obtained simultaneously from a fluid-filled catheter. The tail-cuff was pressurized to suprasystolic levels to completely occlude the tail artery and then released gradually. The pressure at which the tail flow reappeared was recorded as systolic and the pressure at which the tail flow became continuous was recorded as diastolic. Regression analysis of tail-cuff pressures over catheter pressures obtained from healthy mice (n = 16) showed a high degree of association (r(sys) = 0.95, r(dia) = 0.94, both at p < 0.001). Bland-Altman analysis showed good agreement between the two methods, with a mean difference of -13 ( +/- 12 SD) mmHg and 3 ( +/- 10 SD) mmHg in the systolic (58 to 250 mmHg) and diastolic (48 to 178 mmHg) pressure measurements, respectively. Bland-Altman plots of tail-cuff blood pressures of a second group of mice (n = 20) showed good agreement between repeated measurements obtained on the same day, but had higher variability between measurements made on different days.
Anilkumar K Reddy; George E Taffet; Sridhar Madala; Lloyd H Michael; Mark L Entman; Craig J Hartley
Related Documents :
21931069 - Upregulation of inducible nitric oxide synthase contributes to attenuated cutaneous vas...
23953399 - Tyrosine/tyrosinate fluorescence at 700mpa: a pressure unfolding study of chicken ovomu...
23300969 - High altitude bird migration at temperate latitudes: a synoptic perspective on wind ass...
8716299 - A comparison of two noninvasive methods of blood pressure measurement in the triage area.
3896199 - Assessment of aortoiliac disease using hemodynamic measures.
4018039 - Auscultatory measurement of arterial pressure during anaesthesia: a reassessment of kor...
22865479 - Weight gains and increased blood pressure in outpatient hemodialysis patients due to ch...
7940139 - Visual and blood flow responses in low-tension glaucoma.
10431999 - Left atrial systolic time interval in hyperthyroidism.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Validation Studies    
Journal Detail:
Title:  Ultrasound in medicine & biology     Volume:  29     ISSN:  0301-5629     ISO Abbreviation:  Ultrasound Med Biol     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-04-22     Completed Date:  2003-07-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0410553     Medline TA:  Ultrasound Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  379-85     Citation Subset:  IM    
Section of Cardiovascular Sciences, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blood Pressure Determination / methods*
Mice, Inbred C57BL
Reproducibility of Results
Tail / blood supply,  ultrasonography
Ultrasonography, Doppler, Pulsed / instrumentation,  methods*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Measurement of vertical alignment of metatarsal heads using a novel ultrasonographic device.
Next Document:  Carotid artery wall motion estimated from B-mode ultrasound using region tracking and block matching...