Document Detail

Noninvasive blood pressure measurement in mice using pulsed Doppler ultrasound.
MedLine Citation:
PMID:  12706189     Owner:  NLM     Status:  MEDLINE    
Existing tail-cuff pressure devices for mice use tail flow sensors that measure only systolic and mean pressure. We developed a method to obtain systolic and diastolic pressure in mice using a pulsed Doppler flow velocity sensor and a tail-cuff and validated the method against pressure signals obtained simultaneously from a fluid-filled catheter. The tail-cuff was pressurized to suprasystolic levels to completely occlude the tail artery and then released gradually. The pressure at which the tail flow reappeared was recorded as systolic and the pressure at which the tail flow became continuous was recorded as diastolic. Regression analysis of tail-cuff pressures over catheter pressures obtained from healthy mice (n = 16) showed a high degree of association (r(sys) = 0.95, r(dia) = 0.94, both at p < 0.001). Bland-Altman analysis showed good agreement between the two methods, with a mean difference of -13 ( +/- 12 SD) mmHg and 3 ( +/- 10 SD) mmHg in the systolic (58 to 250 mmHg) and diastolic (48 to 178 mmHg) pressure measurements, respectively. Bland-Altman plots of tail-cuff blood pressures of a second group of mice (n = 20) showed good agreement between repeated measurements obtained on the same day, but had higher variability between measurements made on different days.
Anilkumar K Reddy; George E Taffet; Sridhar Madala; Lloyd H Michael; Mark L Entman; Craig J Hartley
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Validation Studies    
Journal Detail:
Title:  Ultrasound in medicine & biology     Volume:  29     ISSN:  0301-5629     ISO Abbreviation:  Ultrasound Med Biol     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-04-22     Completed Date:  2003-07-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0410553     Medline TA:  Ultrasound Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  379-85     Citation Subset:  IM    
Section of Cardiovascular Sciences, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
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MeSH Terms
Blood Pressure Determination / methods*
Mice, Inbred C57BL
Reproducibility of Results
Tail / blood supply,  ultrasonography
Ultrasonography, Doppler, Pulsed / instrumentation,  methods*
Grant Support

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