Document Detail

Noninvasive imaging of the meiotic spindle of in vivo matured oocytes from infertile women with endometriosis.
MedLine Citation:
PMID:  22991379     Owner:  NLM     Status:  MEDLINE    
The objectives of this prospective study were to evaluate the nuclear maturation stage and the presence and location of meiotic spindles of in vivo matured oocytes from infertile women with and without endometriosis (male or tubal causes of infertility) undergoing stimulated cycles for intracytoplasmic sperm injection (ICSI). We also compared the ICSI outcomes among groups. We analyzed the meiotic spindles of oocytes from 36 patients with endometriosis I/II, 24 with endometriosis III/IV, and 60 without endometriosis (male or tubal causes of infertility). The oocytes were imaged using polarization microscopy. There were no differences in the number of oocytes in telophase I (mean [standard deviation]: 0.1 [0.5], 0.2 [0.4], and 0.2 [0.5], respectively, in the endometriosis I/II, endometriosis III/IV, and control groups), in metaphase II with visible spindles (4.2 [2.5], 3.1 [2.0], and 3.6 [2.2], respectively, in the endometriosis I/II, endometriosis III/IV, and control groups), and in spindle location among groups. We can conclude from this study that noninvasive analysis of spindles from in vivo matured oocytes of infertile patients with endometriosis did not demonstrate significant differences in terms of the nuclear maturation stage, the percentage of oocytes in metaphase II with visible spindles, and the spindle localization when compared to the control group. However, it is important to state that there are no studies evaluating the accuracy of polarization microscopy for the detection of meiotic anomalies in human oocytes, which would need to be better evaluated in future studies using an appropriate methodology.
Luciana A Dib; Maria C P M Araújo; Roberta Cristina Giorgenon; Gustavo S Romão; Rui A Ferriani; Paula A Navarro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-18
Journal Detail:
Title:  Reproductive sciences (Thousand Oaks, Calif.)     Volume:  20     ISSN:  1933-7205     ISO Abbreviation:  Reprod Sci     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-15     Completed Date:  2014-05-19     Revised Date:  2014-07-02    
Medline Journal Info:
Nlm Unique ID:  101291249     Medline TA:  Reprod Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  456-62     Citation Subset:  IM    
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MeSH Terms
Case-Control Studies
Endometriosis / diagnosis*,  epidemiology
Infertility, Female / diagnosis*,  epidemiology
Oocyte Retrieval / methods*
Oocytes / physiology*
Prospective Studies
Spindle Apparatus / physiology*

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