| Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice. | |
| | |
MedLine Citation:
|
PMID: 20508727 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
CD8+ T cells are an essential component of an effective host immune response to tumors and viral infections. Genetic immunization is particularly suitable for inducing CTL responses, because the encoded proteins enter the MHC class I processing pathway through either transgene expression or cross-presentation. In order to compare the efficiency and persistence of immune response induced by genetic vaccines, BALB/c mice were immunized either twice intramuscularly with DNA plasmid expressing a codon-optimized HIV-1 gp120 Envelope sequence together with murine GM-CSF sequence or with a single immunization using an integrase defective lentiviral vector (IDLV) expressing the same proteins. Results strongly indicated that the schedule based on IDLV vaccine was more efficient in inducing specific immune response, as evaluated three months after the last immunization by IFNgamma ELISPOT in both splenocytes and bone marrow- (BM-) derived cells, chromium release assay in splenocytes, and antibody detection in sera. In addition, IDLV immunization induced high frequency of polyfunctional CD8+ T cells able to simultaneously produce IFNgamma, TNFalpha, and IL2. |
| | |
Authors:
|
Donatella R M Negri; Zuleika Michelini; Silvia Baroncelli; Massimo Spada; Silvia Vendetti; Roberta Bona; Pasqualina Leone; Mary E Klotman; Andrea Cara |
Related Documents
:
|
7720087 - Systemic treatment with interleukin-4 induces regression of pulmonary metastases in a m... 19595407 - Rsv replication is attenuated by counteracting expression of the suppressor of cytokine... 10415607 - Immune regulatory properties of corticosteroids: prednisone induces apoptosis of human ... 16682497 - Plasmacytoid dendritic cells inhibit pulmonary immunopathology and promote clearance of... 19713447 - Bone marrow stromal cells attenuate lung injury in a murine model of neonatal chronic l... 12752677 - Apoptosis and transplantation tolerance. |
Publication Detail:
|
Type: Journal Article Date: 2010-05-19 |
Journal Detail:
|
Title: Journal of biomedicine & biotechnology Volume: 2010 ISSN: 1110-7251 ISO Abbreviation: J. Biomed. Biotechnol. Publication Date: 2010 |
Date Detail:
|
Created Date: 2010-05-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101135740 Medline TA: J Biomed Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 534501 Citation Subset: - |
Affiliation:
|
Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: beta-Thalassemia: HiJAKing Ineffective Erythropoiesis and Iron Overload.
Next Document: Regulation of the induction and function of cytotoxic T lymphocytes by natural killer T cell.