Document Detail


Noninstrumented enzyme-linked immunosorbant assay for detection of early pregnancy in macaques.
MedLine Citation:
PMID:  11329168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A practical, noninstrumented enzyme-linked immunosorbant assay (NELISA) for the measurement of urinary monkey chorionic gonadotropin (mCG) has been developed for the detection of early pregnancy in macaque monkeys for use in both the laboratory and the field. Five rhesus monkeys (Macaca mulatta) and six crab-eating monkeys (Macaca fascicularis) were tested for the presence of mCG in urine on gestational days (GDs) 12 to 35. The mCG NELISA detected pregnancy as early as GD 14, with an average earliest detection at GD 16.5 +/- 1.4 (n = 11). Out of 90 tests, 27 false-negative and zero false-positive tests were obtained, for an accuracy of 70.0%. Without the aid of a spectrophotometer, the presence of mCG in pregnant monkey samples was indicated by a dark green color change. Nonpregnant monkey urine samples, on the other hand, exhibited no color change. These findings suggest that the simple, economical, and reliable urinary mCG NELISA may be useful for diagnosing early pregnancy in these and related species. Because capture and restraint are unnecessary for collecting urine samples, the mCG NELISA has widespread potential for confined and free-ranging animals.
Authors:
K Shimizu; P N Lohstroh; L S Laughlin; N A Gee; H Todd; S E Shideler; B L Lasley
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of primatology     Volume:  54     ISSN:  0275-2565     ISO Abbreviation:  Am. J. Primatol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-04-30     Completed Date:  2001-06-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8108949     Medline TA:  Am J Primatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  57-62     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Affiliation:
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan. shimizu@pri.kyoto-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Laboratory
Animals, Wild
Chorionic Gonadotropin / immunology,  urine*
Enzyme-Linked Immunosorbent Assay / methods,  veterinary*
Female
Macaca mulatta / physiology*
Pregnancy
Pregnancy Tests, Immunologic / methods,  veterinary*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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