Document Detail


Nonhomologous recombination in the parvovirus chromosome: role for a CTATTTCT motif.
MedLine Citation:
PMID:  3023957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanism of nonhomologous recombination in murine cells infected with the parvovirus minute virus of mice (MVM) has been investigated by analysis of DNA sequences at recombination junctions in naturally occurring deletion variants of the virus. We report here that nonhomologous recombination in the MVM chromosome is characterized by short homologies, by insertion at recombination junctions of foreign DNA sequences that are enriched for preferred eucaryotic topoisomerase I cleavage sites, and by an association with a common DNA sequence motif of the type 5'-CTATTTCT-3'. Additional analyses of broken MVM chromosomes provided evidence for specific enzymatic cleavage within 5'-CTTATC-3' and 5'-CTATTC-3' sequences. The results indicate that the 5'-CTATTTCT-3' motif is an important genetic element for nonhomologous recombination in the parvovirus chromosome.
Authors:
A Hogan; E A Faust
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  6     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1986 Aug 
Date Detail:
Created Date:  1987-01-16     Completed Date:  1987-01-16     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3005-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Chromosome Deletion
Chromosomes*
DNA Topoisomerases, Type I / metabolism
DNA, Viral / analysis
Mice
Minute virus of mice / genetics*
Parvoviridae / genetics*
Recombination, Genetic*
Chemical
Reg. No./Substance:
0/DNA, Viral; EC 5.99.1.2/DNA Topoisomerases, Type I
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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