Document Detail


Nonhemolytic strains of Listeria monocytogenes detected in milk products using VIDAS immunoassay kit.
MedLine Citation:
PMID:  9636989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In December 1995 detection of Listeria monocytogenes Sv 1/2a in milk products that were routinely sampled for investigation at the Austrian Federal Food Inspection Laboratory (Vienna) led the food manufacturer in question to withdraw his product from the market. While one of seven Listeria strains isolated from this food product using "VIDAS L. monocytogenes kit" was undoubtedly L. monocyotogenes, six strains were nonhemolytic. In classical bacteriology haemolysin is used as an important phenotypic property to differentiate L. monocytogenes from the apathogenic L. innocua species. Species identification by PCR and by Western blotting confirmed that all nonhemolytic strains were indeed L. monocytogenes. Our confirmation of nonhemolytic isolates as true L. monocytogenes strains underlines the considerable potential of the VIDAS system as a specific immunological easy-to-use and automated test kit for detection of L. monocytogenes in food products. Whether or not the presence of non-hemolytic Listeria monocytogenes in food products justifies legal actions should be addressed by the proper authorities.
Authors:
F Allerberger; M Dierich; G Petranyi; M Lalic; A Bubert
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zentralblatt für Hygiene und Umweltmedizin = International journal of hygiene and environmental medicine     Volume:  200     ISSN:  0934-8859     ISO Abbreviation:  Zentralbl Hyg Umweltmed     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1998-07-24     Completed Date:  1998-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8912563     Medline TA:  Zentralbl Hyg Umweltmed     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  189-95     Citation Subset:  IM    
Affiliation:
Bundesstaatliche bakteriologische-serologische Untersuchungsanstalt, Innsbruck.
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MeSH Terms
Descriptor/Qualifier:
Austria
Blotting, Western
Dairy Products / microbiology*,  standards
Hemolysin Proteins / analysis
Listeria monocytogenes / classification,  isolation & purification*,  pathogenicity
Polymerase Chain Reaction / methods
Reagent Kits, Diagnostic
Chemical
Reg. No./Substance:
0/Hemolysin Proteins; 0/Reagent Kits, Diagnostic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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