| Nonenzymatic augmentation of lactate transport via monocarboxylate transporter isoform 4 by carbonic anhydrase II. | |
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MedLine Citation:
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PMID: 20300744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monocarboxylate transporters (MCTs) are carriers of high-energy metabolites like lactate and pyruvate, and different MCT isoforms are expressed in a wide range of cells and tissues. Transport activity of MCT isoform 1 (MCT1), heterologously expressed in Xenopus oocytes, has previously been shown to be supported by carbonic anhydrase II (CAII) in a noncatalytic manner. In the present study, we investigated possible interactions of CAII with MCT4, expressed in Xenopus oocytes. MCT4 transport activity is enhanced both by injected and by coexpressed CAII, similar to MCT1, with the highest augmentation at low extracellular pH and low lactate concentrations. CAII-induced augmentation in MCT4 transport activity is independent from the enzyme's catalytic function, as shown by application of the CA inhibitor ethoxyzolamide and by coexpression of MCT4 with the catalytically inactive mutant CAII-V143Y. |
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Authors:
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Holger M Becker; Michael Klier; Joachim W Deitmer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-19 |
Journal Detail:
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Title: The Journal of membrane biology Volume: 234 ISSN: 1432-1424 ISO Abbreviation: J. Membr. Biol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-02 Completed Date: 2010-06-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0211301 Medline TA: J Membr Biol Country: United States |
Other Details:
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Languages: eng Pagination: 125-35 Citation Subset: IM |
Affiliation:
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Arbeitsgruppe Zoologie/Membrantransport, FB Biologie, TU Kaiserslautern, P.O. Box 3049, 67653, Kaiserslautern, Germany. h.becker@biologie.uni-kl.de |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbonic Anhydrase II / metabolism* Carbonic Anhydrase Inhibitors / pharmacology Catalysis Ethoxzolamide / pharmacology Humans Hydrogen-Ion Concentration Lactic Acid / metabolism* Monocarboxylic Acid Transporters / metabolism* Muscle Proteins / metabolism* Oocytes / metabolism Rats Xenopus laevis |
| Chemical | |
Reg. No./Substance:
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0/Carbonic Anhydrase Inhibitors; 0/MCT4 protein, rat; 0/Monocarboxylic Acid Transporters; 0/Muscle Proteins; 452-35-7/Ethoxzolamide; 50-21-5/Lactic Acid; EC 4.2.1.-/Carbonic Anhydrase II |
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