Document Detail


Noncovalent association of heavy and light chains of human immunoglobulins. III. Specific interactions between VH and VL.
MedLine Citation:
PMID:  6806377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mildly reduced monoclonal human IgM proteins have been cleaved at cysteinyl residues to give VH fragments after S-cyanylation with 2-nitro-5-thiocyanobenzoic acid. The noncovalent interaction between the VH fragments and autologous kappa-chains was studied by ultraviolet difference spectroscopy and circular dichroism. A bimolecular complex was formed with an association constant in excess of 10(7) M-1 at 23 degrees C. Complex formation was accompanied by burial of tryptophan and tyrosine side-chains. In contrast to the studies with autologous species, the VH fragments did not associate with heterologous kappa-chains as judged both by difference spectroscopy and gel filtration using radiolabeled VH fragments. This specificity in the association between VH and VL has been attributed to interactions contributed to by residues in the third hypervariable region of VH encoded by the DH and JH genes.
Authors:
C Horne; M Klein; I Polidoulis; K J Dorrington
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  129     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1982 Aug 
Date Detail:
Created Date:  1982-08-26     Completed Date:  1982-08-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  660-4     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Binding Sites, Antibody*
Chemical Phenomena
Chemistry
Chromatography, Gel
Circular Dichroism
Humans
Immunoglobulin Heavy Chains* / genetics
Immunoglobulin Light Chains*
Immunoglobulin Variable Region* / genetics
Immunoglobulin kappa-Chains
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Binding Sites, Antibody; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Light Chains; 0/Immunoglobulin Variable Region; 0/Immunoglobulin kappa-Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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