Document Detail


Noncompetitive inhibition by camphor of nicotinic acetylcholine receptors.
MedLine Citation:
PMID:  11274963     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of camphor, a monoterpenoid, on catecholamine secretion was investigated in bovine adrenal chromaffin cells. Camphor inhibited [3H]norepinephrine ([3H]NE) secretion induced by a nicotinic acetylcholine receptor (nAChR) agonist, 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP), with a half-maximal inhibitory concentration (IC50) of 70 +/- 12 microM. In addition, camphor inhibited the rise in cytosolic calcium ([Ca2+]i) and sodium ([Na+]i) induced by DMPP with IC50 values of 88 +/- 32 and 19 +/- 2 microM, respectively, suggesting that the activity of nAChRs is also inhibited by camphor. On the other hand, binding of [3H]nicotine to nAChRs was not affected by camphor. [Ca2+]i increases induced by high K+, veratridine, and bradykinin were not affected by camphor. The data suggest that camphor specifically inhibits catecholamine secretion by blocking nAChRs without affecting agonist binding.
Authors:
T J Park; H K Seo; B J Kang; K T Kim
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical pharmacology     Volume:  61     ISSN:  0006-2952     ISO Abbreviation:  Biochem. Pharmacol.     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-03-29     Completed Date:  2001-04-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0101032     Medline TA:  Biochem Pharmacol     Country:  England    
Other Details:
Languages:  eng     Pagination:  787-93     Citation Subset:  IM    
Affiliation:
Department of Life Science, Division of Molecular and Life Science, Pohang University of Science and Technology, San 31, Hyoja Dong, 790-784, Pohang, Korea. ktk@postech.ac.kr
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Infective Agents, Local / pharmacology
Binding Sites / drug effects
Calcium / metabolism
Camphor / pharmacology*
Catecholamines / secretion
Cattle
Chromaffin Cells / drug effects*,  metabolism
Dimethylphenylpiperazinium Iodide / pharmacology
Drug Interactions
Nicotine / pharmacology
Nicotinic Agonists / pharmacology
Nicotinic Antagonists / pharmacology*
Norepinephrine / secretion
Receptors, Nicotinic / drug effects,  metabolism*
Sodium / metabolism
Time Factors
Tritium
Chemical
Reg. No./Substance:
0/Anti-Infective Agents, Local; 0/Catecholamines; 0/Nicotinic Agonists; 0/Nicotinic Antagonists; 0/Receptors, Nicotinic; 10028-17-8/Tritium; 51-41-2/Norepinephrine; 54-11-5/Nicotine; 54-77-3/Dimethylphenylpiperazinium Iodide; 7440-23-5/Sodium; 7440-70-2/Calcium; 76-22-2/Camphor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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