Document Detail

Noncoding transcription controls downstream promoters to regulate T-cell receptor alpha recombination.
MedLine Citation:
PMID:  17882258     Owner:  NLM     Status:  MEDLINE    
The T early alpha (TEA) promoter in the murine Tcra locus generates noncoding transcripts that extend across the 65 kb Jalpha array. Here, we have analyzed the significance of TEA transcription for Tcra locus regulation through the targeted introduction of a transcription terminator downstream of the TEA promoter. We demonstrate that noncoding transcription driven by this single promoter can instruct both positively and negatively the activity of downstream Jalpha promoters, and can similarly instruct alterations in Jalpha chromatin structure and Jalpha recombination. TEA transcription activates promoters associated with relatively proximal Jalpha segments and stimulates histone acetylation, histone methylation and chromatin accessibility in this region. In contrast, at more distal locations, TEA transcription inhibits promoter activity through transcriptional interference and suppresses chromatin accessibility. In combination, these effects target initial Valpha-to-Jalpha recombination to TEA-proximal Jalpha segments and promote the ordered usage of the Jalpha array. The ability of TEA transcription to coordinate the activity of multiple downstream promoters maximizes the biological potential of the Jalpha array and diversifies the Tcra repertoire.
Iratxe Abarrategui; Michael S Krangel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-09-20
Journal Detail:
Title:  The EMBO journal     Volume:  26     ISSN:  1460-2075     ISO Abbreviation:  EMBO J.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-17     Completed Date:  2007-12-11     Revised Date:  2013-03-28    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  4380-90     Citation Subset:  IM    
Department of Immunology, Duke University Medical Center, Durham, NC, USA.
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MeSH Terms
Base Sequence
Chromatin / chemistry
Histones / chemistry
Mice, Transgenic
Models, Genetic
Molecular Sequence Data
Promoter Regions, Genetic
Receptors, Antigen, T-Cell / metabolism
Receptors, Antigen, T-Cell, alpha-beta / genetics*,  physiology*
Recombination, Genetic*
T-Lymphocytes / metabolism
Transcription, Genetic*
Grant Support
Reg. No./Substance:
0/Chromatin; 0/Histones; 0/Receptors, Antigen, T-Cell; 0/Receptors, Antigen, T-Cell, alpha-beta

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